Epiblast stem cell-based system reveals reprogramming synergy of germline factors

Astrid Gillich, Siqin Bao, Nils Grabole, Katsuhiko Hayashi, Matthew W.B. Trotter, Vincent Pasque, Erna Magnúsdóttir, M. Azim Surani

研究成果: ジャーナルへの寄稿記事

95 引用 (Scopus)

抄録

Epigenetic reprogramming in early germ cells is critical toward the establishment of totipotency, but investigations of the germline events are intractable. An objective cell culture-based system could provide mechanistic insight on how the key determinants of primordial germ cells (PGCs), including Prdm14, induce reprogramming in germ cells to an epigenetic ground state. Here we show a Prdm14-Klf2 synergistic effect that can accelerate and enhance reversion of mouse epiblast stem cells (epiSCs) to a naive pluripotent state, including X reactivation and DNA demethylation. Notably, Prdm14 alone has little effect on epiSC reversion, but it enhances the competence for reprogramming and potentially PGC specification. Reprogramming of epiSCs by the combinatorial effect of Prdm14-Klf2 involves key epigenetic changes, which might have an analogous role in PGCs. Our study provides a paradigm toward a systematic analysis of how other key genes contribute to complex and dynamic events of reprogramming in the germline.

元の言語英語
ページ(範囲)425-439
ページ数15
ジャーナルCell stem cell
10
発行部数4
DOI
出版物ステータス出版済み - 4 6 2012

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Germ Layers
Germ Cells
Stem Cells
Epigenomics
Mental Competency
Cell Culture Techniques
DNA
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Genetics
  • Cell Biology

これを引用

Gillich, A., Bao, S., Grabole, N., Hayashi, K., Trotter, M. W. B., Pasque, V., ... Surani, M. A. (2012). Epiblast stem cell-based system reveals reprogramming synergy of germline factors. Cell stem cell, 10(4), 425-439. https://doi.org/10.1016/j.stem.2012.01.020

Epiblast stem cell-based system reveals reprogramming synergy of germline factors. / Gillich, Astrid; Bao, Siqin; Grabole, Nils; Hayashi, Katsuhiko; Trotter, Matthew W.B.; Pasque, Vincent; Magnúsdóttir, Erna; Surani, M. Azim.

:: Cell stem cell, 巻 10, 番号 4, 06.04.2012, p. 425-439.

研究成果: ジャーナルへの寄稿記事

Gillich, A, Bao, S, Grabole, N, Hayashi, K, Trotter, MWB, Pasque, V, Magnúsdóttir, E & Surani, MA 2012, 'Epiblast stem cell-based system reveals reprogramming synergy of germline factors', Cell stem cell, 巻. 10, 番号 4, pp. 425-439. https://doi.org/10.1016/j.stem.2012.01.020
Gillich, Astrid ; Bao, Siqin ; Grabole, Nils ; Hayashi, Katsuhiko ; Trotter, Matthew W.B. ; Pasque, Vincent ; Magnúsdóttir, Erna ; Surani, M. Azim. / Epiblast stem cell-based system reveals reprogramming synergy of germline factors. :: Cell stem cell. 2012 ; 巻 10, 番号 4. pp. 425-439.
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