Epigallocatechin-3-gallate, a polyphenol component of green tea, suppresses both collagen production and collagenase activity in hepatic stellate cells.

Makoto Nakamuta, Nobuhiko Higashi, Motoyuki Kohjima, Marie Fukushima, Satoshi Ohta, Kazuhiro Kotoh, Naoya Kobayashi, Munechika Enjoji

研究成果: ジャーナルへの寄稿記事

61 引用 (Scopus)

抄録

Catechins such as epigallocatechin-3-gallate (EGCG), epicatechin-3-gallate (ECG), and epigallocatechin (EGC) are polyphenol components of green tea. EGCG is the major component and has been reported to possess a wide range of biological properties including anti-fibrogenic activity. In hepatic fibrosis, activated hepatic stellate cells (HSCs) play a central role. In this study, we investigated the effect of catechins, including EGCG, on collagen production and collagenase activity in rat primary HSCs and activated human HSC-derived TWNT-4 cells. EGCG (50 microM) suppressed type I collagen production in rat HSCs more than ECG (50 microM) did; however, EGC (50 microM) did not show suppressive effects. EGCG also inhibited both collagen production and collagenase activity (active matrix metalloproteinase-1 [MMP-1]) in a dose-dependent manner, but did not affect the tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) production in TWNT-4 cells. Real-time PCR unexpectedly revealed that EGCG enhanced the transcription of type I collagen and TIMP-1, but did not affect the transcription of alpha-smooth muscle actin (alpha-SMA), and reduced the transcription MMP-1 in TWNT-4 cells. These findings demonstrated that EGCG inhibited collagen production regardless of enhanced collagen transcription and suppressed collagenase activity, and suggested that EGCG might have therapeutic potential for liver fibrosis.

元の言語英語
ページ(範囲)677-681
ページ数5
ジャーナルInternational journal of molecular medicine
16
発行部数4
出版物ステータス出版済み - 1 1 2005

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Hepatic Stellate Cells
Polyphenols
Collagenases
Tea
Collagen
Matrix Metalloproteinase 1
Tissue Inhibitor of Metalloproteinase-1
Catechin
Collagen Type I
epigallocatechin gallate
Liver Cirrhosis
Smooth Muscle
Actins
Real-Time Polymerase Chain Reaction
Fibrosis
Liver

All Science Journal Classification (ASJC) codes

  • Genetics

これを引用

Epigallocatechin-3-gallate, a polyphenol component of green tea, suppresses both collagen production and collagenase activity in hepatic stellate cells. / Nakamuta, Makoto; Higashi, Nobuhiko; Kohjima, Motoyuki; Fukushima, Marie; Ohta, Satoshi; Kotoh, Kazuhiro; Kobayashi, Naoya; Enjoji, Munechika.

:: International journal of molecular medicine, 巻 16, 番号 4, 01.01.2005, p. 677-681.

研究成果: ジャーナルへの寄稿記事

Nakamuta, Makoto ; Higashi, Nobuhiko ; Kohjima, Motoyuki ; Fukushima, Marie ; Ohta, Satoshi ; Kotoh, Kazuhiro ; Kobayashi, Naoya ; Enjoji, Munechika. / Epigallocatechin-3-gallate, a polyphenol component of green tea, suppresses both collagen production and collagenase activity in hepatic stellate cells. :: International journal of molecular medicine. 2005 ; 巻 16, 番号 4. pp. 677-681.
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abstract = "Catechins such as epigallocatechin-3-gallate (EGCG), epicatechin-3-gallate (ECG), and epigallocatechin (EGC) are polyphenol components of green tea. EGCG is the major component and has been reported to possess a wide range of biological properties including anti-fibrogenic activity. In hepatic fibrosis, activated hepatic stellate cells (HSCs) play a central role. In this study, we investigated the effect of catechins, including EGCG, on collagen production and collagenase activity in rat primary HSCs and activated human HSC-derived TWNT-4 cells. EGCG (50 microM) suppressed type I collagen production in rat HSCs more than ECG (50 microM) did; however, EGC (50 microM) did not show suppressive effects. EGCG also inhibited both collagen production and collagenase activity (active matrix metalloproteinase-1 [MMP-1]) in a dose-dependent manner, but did not affect the tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) production in TWNT-4 cells. Real-time PCR unexpectedly revealed that EGCG enhanced the transcription of type I collagen and TIMP-1, but did not affect the transcription of alpha-smooth muscle actin (alpha-SMA), and reduced the transcription MMP-1 in TWNT-4 cells. These findings demonstrated that EGCG inhibited collagen production regardless of enhanced collagen transcription and suppressed collagenase activity, and suggested that EGCG might have therapeutic potential for liver fibrosis.",
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AU - Nakamuta, Makoto

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AU - Kohjima, Motoyuki

AU - Fukushima, Marie

AU - Ohta, Satoshi

AU - Kotoh, Kazuhiro

AU - Kobayashi, Naoya

AU - Enjoji, Munechika

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