Epigallocatechin-3-O-gallate induces acid sphingomyelinase activation through activation of phospholipase C

Jaehoon Bae, Motofumi Kumazoe, Chieri Takeuchi, Shiori Hidaka, Yoshinori Fujimura, Hirofumi Tachibana

研究成果: ジャーナルへの寄稿記事

抄録

Epigallocatechin-3-O-gallate (EGCG)-induced cyclic guanosine monophosphate (cGMP) plays a crucial role in EGCG-induced cell death in various types of cancer cells. However, little is known regarding the early molecular events after cGMP induction. In this study, we showed that cGMP induction is sufficient to induce the phosphorylation of protein kinase C delta (PKCδ) at Ser664, the crucial kinase for EGCG-induced activation of acid sphingomyelinase (ASM). Using a chemical inhibitor library, we revealed that the inhibitors of the negative regulators of diacylglycerol strongly increase the effect of EGCG. We also showed that EGCG treatment increased phospholipase C (PLC) activity, and the same results were obtained with cGMP inducer treatment. EGCG-induced ASM activation was completely suppressed by pharmacological inhibition of PLC. Collectively, EGCG-induced cGMP activated the cGMP/PLC/PKCδ/ASM signaling axis in multiple myeloma cells.

元の言語英語
ページ(範囲)186-191
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
520
発行部数1
DOI
出版物ステータス出版済み - 11 26 2019

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Sphingomyelin Phosphodiesterase
Type C Phospholipases
Cyclic GMP
Chemical activation
Acids
Protein Kinase C-delta
Small Molecule Libraries
Phosphorylation
Diglycerides
Cell death
epigallocatechin gallate
Multiple Myeloma
Cell Death
Phosphotransferases
Cells
Pharmacology

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Epigallocatechin-3-O-gallate induces acid sphingomyelinase activation through activation of phospholipase C. / Bae, Jaehoon; Kumazoe, Motofumi; Takeuchi, Chieri; Hidaka, Shiori; Fujimura, Yoshinori; Tachibana, Hirofumi.

:: Biochemical and Biophysical Research Communications, 巻 520, 番号 1, 26.11.2019, p. 186-191.

研究成果: ジャーナルへの寄稿記事

Bae, Jaehoon ; Kumazoe, Motofumi ; Takeuchi, Chieri ; Hidaka, Shiori ; Fujimura, Yoshinori ; Tachibana, Hirofumi. / Epigallocatechin-3-O-gallate induces acid sphingomyelinase activation through activation of phospholipase C. :: Biochemical and Biophysical Research Communications. 2019 ; 巻 520, 番号 1. pp. 186-191.
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abstract = "Epigallocatechin-3-O-gallate (EGCG)-induced cyclic guanosine monophosphate (cGMP) plays a crucial role in EGCG-induced cell death in various types of cancer cells. However, little is known regarding the early molecular events after cGMP induction. In this study, we showed that cGMP induction is sufficient to induce the phosphorylation of protein kinase C delta (PKCδ) at Ser664, the crucial kinase for EGCG-induced activation of acid sphingomyelinase (ASM). Using a chemical inhibitor library, we revealed that the inhibitors of the negative regulators of diacylglycerol strongly increase the effect of EGCG. We also showed that EGCG treatment increased phospholipase C (PLC) activity, and the same results were obtained with cGMP inducer treatment. EGCG-induced ASM activation was completely suppressed by pharmacological inhibition of PLC. Collectively, EGCG-induced cGMP activated the cGMP/PLC/PKCδ/ASM signaling axis in multiple myeloma cells.",
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AU - Takeuchi, Chieri

AU - Hidaka, Shiori

AU - Fujimura, Yoshinori

AU - Tachibana, Hirofumi

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