Epigenetic analysis of a single DNA molecule by microfluidic device with quantum dot

Tatsuki Sano, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

研究成果: 書籍/レポート タイプへの寄稿会議への寄与

1 被引用数 (Scopus)

抄録

We report a rapid and simple epigenetic analysis based on microfluidic single DNA molecular methylation detection. We developed a microfluidic device to stretch a single DNA molecule and subsequent detection of a methylated DNA base by quantum dot (QD)-immobilized methyl binding domain (MBD) protein. A QD-MBD complex specifically bound to a methylated base of stretched DNA and was detected by a total internal reflection fluorescence microscope (TIRFM). Our method enables us to detect a number of methylation sites and identify the positions methylated simultaneously in the simple and rapid manner compared with the conventional methods.

本文言語英語
ホスト出版物のタイトル15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
ページ299-301
ページ数3
1
出版ステータス出版済み - 12月 1 2011
外部発表はい
イベント15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, 米国
継続期間: 10月 2 201110月 6 2011

その他

その他15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
国/地域米国
CitySeattle, WA
Period10/2/1110/6/11

!!!All Science Journal Classification (ASJC) codes

  • 制御およびシステム工学

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