Epithelial-mesenchymal transition is activated in CD44-positive malignant ascites tumor cells of gastrointestinal cancer

Michitaka Nakano, Mamoru Ito, Risa Tanaka, hiroshi ariyama, Kenji Mitsugi, Akitaka Makiyama, Keita Uchino, Taito Esaki, Nobuhiro Tsuruta, Fumiyasu Hanamura, Kyoko Yamaguchi, Yuta Okumura, Kosuke Sagara, Kotoe Takayoshi, Kenta Nio, Kenji Tsuchihashi, Shingo Tamura, Hozumi Shimokawa, Shuji Arita, Kohta Miyawaki & 3 others Hitoshi Kusaba, Koichi Akashi, Eishi Baba

研究成果: ジャーナルへの寄稿記事

抄録

Disseminated cancer cells in malignant ascites possess unique properties that differ from primary tumors. However, the biological features of ascites tumor cells (ATC) have not been fully investigated. By analyzing ascites fluid from 65 gastrointestinal cancer patients, the distinguishing characteristics of ATC were identified. High frequency of CD44+ cells was observed in ATC using flow cytometry (n = 48). Multiplex quantitative PCR (n = 15) showed higher gene expression of epithelial-mesenchymal transition (EMT)-related genes and transforming growth factor beta (TGF-beta)-related genes in ATC than in the primary tissues. Immunohistochemistry (n = 10) showed that ATC also had much higher expression of phosphorylated SMAD2 than that in the corresponding primary tissues. TGF-beta 1 was detected in all cases of malignant ascites by enzyme-linked immunoassay (n = 38), suggesting the possible interaction of ATC and the ascites microenvironment. In vitro experiments revealed that these ATC properties were maintained by TGF-beta 1 in cultured ATC(n = 3). Here, we showed that ATCrevealed high frequencies of CD44 and possessed distinct EMT features from primary tissues that were mainly maintained by TGF-beta 1 in the ascites.

元の言語英語
ページ(範囲)3461-3470
ページ数10
ジャーナルCancer Science
109
発行部数11
DOI
出版物ステータス出版済み - 11 1 2018

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Gastrointestinal Neoplasms
Epithelial-Mesenchymal Transition
Ascites
Neoplasms
Transforming Growth Factor beta
Cultured Tumor Cells
Cellular Microenvironment
Multiplex Polymerase Chain Reaction
Immunoenzyme Techniques
Genes
Flow Cytometry

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

これを引用

Epithelial-mesenchymal transition is activated in CD44-positive malignant ascites tumor cells of gastrointestinal cancer. / Nakano, Michitaka; Ito, Mamoru; Tanaka, Risa; ariyama, hiroshi; Mitsugi, Kenji; Makiyama, Akitaka; Uchino, Keita; Esaki, Taito; Tsuruta, Nobuhiro; Hanamura, Fumiyasu; Yamaguchi, Kyoko; Okumura, Yuta; Sagara, Kosuke; Takayoshi, Kotoe; Nio, Kenta; Tsuchihashi, Kenji; Tamura, Shingo; Shimokawa, Hozumi; Arita, Shuji; Miyawaki, Kohta; Kusaba, Hitoshi; Akashi, Koichi; Baba, Eishi.

:: Cancer Science, 巻 109, 番号 11, 01.11.2018, p. 3461-3470.

研究成果: ジャーナルへの寄稿記事

Nakano, M, Ito, M, Tanaka, R, ariyama, H, Mitsugi, K, Makiyama, A, Uchino, K, Esaki, T, Tsuruta, N, Hanamura, F, Yamaguchi, K, Okumura, Y, Sagara, K, Takayoshi, K, Nio, K, Tsuchihashi, K, Tamura, S, Shimokawa, H, Arita, S, Miyawaki, K, Kusaba, H, Akashi, K & Baba, E 2018, 'Epithelial-mesenchymal transition is activated in CD44-positive malignant ascites tumor cells of gastrointestinal cancer', Cancer Science, 巻. 109, 番号 11, pp. 3461-3470. https://doi.org/10.1111/cas.13777
Nakano, Michitaka ; Ito, Mamoru ; Tanaka, Risa ; ariyama, hiroshi ; Mitsugi, Kenji ; Makiyama, Akitaka ; Uchino, Keita ; Esaki, Taito ; Tsuruta, Nobuhiro ; Hanamura, Fumiyasu ; Yamaguchi, Kyoko ; Okumura, Yuta ; Sagara, Kosuke ; Takayoshi, Kotoe ; Nio, Kenta ; Tsuchihashi, Kenji ; Tamura, Shingo ; Shimokawa, Hozumi ; Arita, Shuji ; Miyawaki, Kohta ; Kusaba, Hitoshi ; Akashi, Koichi ; Baba, Eishi. / Epithelial-mesenchymal transition is activated in CD44-positive malignant ascites tumor cells of gastrointestinal cancer. :: Cancer Science. 2018 ; 巻 109, 番号 11. pp. 3461-3470.
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abstract = "Disseminated cancer cells in malignant ascites possess unique properties that differ from primary tumors. However, the biological features of ascites tumor cells (ATC) have not been fully investigated. By analyzing ascites fluid from 65 gastrointestinal cancer patients, the distinguishing characteristics of ATC were identified. High frequency of CD44+ cells was observed in ATC using flow cytometry (n = 48). Multiplex quantitative PCR (n = 15) showed higher gene expression of epithelial-mesenchymal transition (EMT)-related genes and transforming growth factor beta (TGF-beta)-related genes in ATC than in the primary tissues. Immunohistochemistry (n = 10) showed that ATC also had much higher expression of phosphorylated SMAD2 than that in the corresponding primary tissues. TGF-beta 1 was detected in all cases of malignant ascites by enzyme-linked immunoassay (n = 38), suggesting the possible interaction of ATC and the ascites microenvironment. In vitro experiments revealed that these ATC properties were maintained by TGF-beta 1 in cultured ATC(n = 3). Here, we showed that ATCrevealed high frequencies of CD44 and possessed distinct EMT features from primary tissues that were mainly maintained by TGF-beta 1 in the ascites.",
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AU - Nakano, Michitaka

AU - Ito, Mamoru

AU - Tanaka, Risa

AU - ariyama, hiroshi

AU - Mitsugi, Kenji

AU - Makiyama, Akitaka

AU - Uchino, Keita

AU - Esaki, Taito

AU - Tsuruta, Nobuhiro

AU - Hanamura, Fumiyasu

AU - Yamaguchi, Kyoko

AU - Okumura, Yuta

AU - Sagara, Kosuke

AU - Takayoshi, Kotoe

AU - Nio, Kenta

AU - Tsuchihashi, Kenji

AU - Tamura, Shingo

AU - Shimokawa, Hozumi

AU - Arita, Shuji

AU - Miyawaki, Kohta

AU - Kusaba, Hitoshi

AU - Akashi, Koichi

AU - Baba, Eishi

PY - 2018/11/1

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N2 - Disseminated cancer cells in malignant ascites possess unique properties that differ from primary tumors. However, the biological features of ascites tumor cells (ATC) have not been fully investigated. By analyzing ascites fluid from 65 gastrointestinal cancer patients, the distinguishing characteristics of ATC were identified. High frequency of CD44+ cells was observed in ATC using flow cytometry (n = 48). Multiplex quantitative PCR (n = 15) showed higher gene expression of epithelial-mesenchymal transition (EMT)-related genes and transforming growth factor beta (TGF-beta)-related genes in ATC than in the primary tissues. Immunohistochemistry (n = 10) showed that ATC also had much higher expression of phosphorylated SMAD2 than that in the corresponding primary tissues. TGF-beta 1 was detected in all cases of malignant ascites by enzyme-linked immunoassay (n = 38), suggesting the possible interaction of ATC and the ascites microenvironment. In vitro experiments revealed that these ATC properties were maintained by TGF-beta 1 in cultured ATC(n = 3). Here, we showed that ATCrevealed high frequencies of CD44 and possessed distinct EMT features from primary tissues that were mainly maintained by TGF-beta 1 in the ascites.

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