EPLIN is a crucial regulator for extrusion of RasV12- transformed cells

Atsuko Ohoka, Mihoko Kajita, Junichi Ikenouchi, Yuta Yako, Sho Kitamoto, Shunsuke Kon, Masaya Ikegawa, Takashi Shimada, Susumu Ishikawa, Yasuyuki Fujita

研究成果: ジャーナルへの寄稿記事

31 引用 (Scopus)

抄録

At the initial stage of carcinogenesis, a mutation occurs in a single cell within a normal epithelial layer. We have previously shown that RasV12-transformed cells are apically extruded from the epithelium when surrounded by normal cells. However, the molecular mechanisms underlying this phenomenon remain elusive. Here, we demonstrate that Cav-1-containing microdomains and EPLIN (also known as LIMA1) are accumulated in RasV12-transformed cells that are surrounded by normal cells. We also show that knockdown of Cav-1 or EPLIN suppresses apical extrusion of RasV12-transformed cells, suggesting their positive role in the elimination of transformed cells from epithelia. EPLIN functions upstream of Cav-1 and affects its enrichment in RasV12-transformed cells that are surrounded by normal cells. Furthermore, EPLIN regulates non-cell-autonomous activation of myosin-II and protein kinase A (PKA) in RasV12-transformed cells. In addition, EPLIN substantially affects the accumulation of filamin A, a vital player in epithelial defense against cancer (EDAC), in the neighboring normal cells, and vice versa. These results indicate that EPLIN is a crucial regulator of the interaction between normal and transformed epithelial cells.

元の言語英語
ページ(範囲)781-789
ページ数9
ジャーナルJournal of Cell Science
128
発行部数4
DOI
出版物ステータス出版済み - 1 1 2015

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Epithelium
Filamins
Myosin Type II
Myosin-Light-Chain Kinase
Cyclic AMP-Dependent Protein Kinases
Carcinogenesis
Epithelial Cells
Mutation
Neoplasms

All Science Journal Classification (ASJC) codes

  • Cell Biology

これを引用

Ohoka, A., Kajita, M., Ikenouchi, J., Yako, Y., Kitamoto, S., Kon, S., ... Fujita, Y. (2015). EPLIN is a crucial regulator for extrusion of RasV12- transformed cells. Journal of Cell Science, 128(4), 781-789. https://doi.org/10.1242/jcs.163113

EPLIN is a crucial regulator for extrusion of RasV12- transformed cells. / Ohoka, Atsuko; Kajita, Mihoko; Ikenouchi, Junichi; Yako, Yuta; Kitamoto, Sho; Kon, Shunsuke; Ikegawa, Masaya; Shimada, Takashi; Ishikawa, Susumu; Fujita, Yasuyuki.

:: Journal of Cell Science, 巻 128, 番号 4, 01.01.2015, p. 781-789.

研究成果: ジャーナルへの寄稿記事

Ohoka, A, Kajita, M, Ikenouchi, J, Yako, Y, Kitamoto, S, Kon, S, Ikegawa, M, Shimada, T, Ishikawa, S & Fujita, Y 2015, 'EPLIN is a crucial regulator for extrusion of RasV12- transformed cells', Journal of Cell Science, 巻. 128, 番号 4, pp. 781-789. https://doi.org/10.1242/jcs.163113
Ohoka, Atsuko ; Kajita, Mihoko ; Ikenouchi, Junichi ; Yako, Yuta ; Kitamoto, Sho ; Kon, Shunsuke ; Ikegawa, Masaya ; Shimada, Takashi ; Ishikawa, Susumu ; Fujita, Yasuyuki. / EPLIN is a crucial regulator for extrusion of RasV12- transformed cells. :: Journal of Cell Science. 2015 ; 巻 128, 番号 4. pp. 781-789.
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AU - Kitamoto, Sho

AU - Kon, Shunsuke

AU - Ikegawa, Masaya

AU - Shimada, Takashi

AU - Ishikawa, Susumu

AU - Fujita, Yasuyuki

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