Erratum to: Quartz-Seq: A highly reproducible and sensitive single-cell RNA sequencing method, reveals non-genetic gene-expression heterogeneity. [Genome Biol. 14, (2013), (R31)]

Yohei Sasagawa, Itoshi Nikaido, Tetsutaro Hayashi, Hiroki Danno, Kenichiro D. Uno, Takeshi Imai, Hiroki R. Ueda

研究成果: ジャーナルへの寄稿コメント/討論

2 引用 (Scopus)

抄録

After publication of our article [1], we noticed some errors. In this manuscript, we amplified cDNA with 41.67 nmol/l RT primer and 70 nmol/l tagging primer for single-cell Quartz-Seq, and not pmol/l as originally stated. In addition, two mathematical expressions were inadvertently omitted. Thus, in the section Whole-transcript amplification for single-cell Quartz-Seq, the following are correct: Immediately after the second centrifugation, 0.8 μl of priming buffer (1.5× PCR buffer with MgCl2 (TaKaRa Bio), 41.67 nmol/l of the RT primer (HPLC-purified; Table 1), 4 U/μl of RNase inhibitor (RNasin Plus; Promega Corp., Madison, WI, USA), and 50 μmol/l dNTPs were added to each tube. And We then added 23 μl of the second-strand buffer (1.09× MightyAmp Buffer v2 (TaKaRa), 70 nmol/l tagging primer (HPLC-purified; Table 1), and 0.054 U/μl MightyAmp DNA polymerase (TaKaRa)) to each tube. In the section Bioinformatics analysis, the equations should appear as follows: The MI is considered the Kullback-Leibler distance from the joint probability density to the product of the marginal probability densities as follows: The MI is always non-negative, symmetric, and equal to 0 only if × and Y are independent. The MI can be represented as a summation of entropies.

元の言語英語
記事番号9
ジャーナルGenome biology
18
発行部数1
DOI
出版物ステータス出版済み - 1 18 2017
外部発表Yes

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RNA Sequence Analysis
Quartz
tagging
quartz
gene expression
RNA
Buffers
buffers
sequence analysis
genome
Genome
Gene Expression
bioinformatics
entropy
inhibitor
amplification
High Pressure Liquid Chromatography
cells
DNA
Magnesium Chloride

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Cell Biology

これを引用

Erratum to : Quartz-Seq: A highly reproducible and sensitive single-cell RNA sequencing method, reveals non-genetic gene-expression heterogeneity. [Genome Biol. 14, (2013), (R31)]. / Sasagawa, Yohei; Nikaido, Itoshi; Hayashi, Tetsutaro; Danno, Hiroki; Uno, Kenichiro D.; Imai, Takeshi; Ueda, Hiroki R.

:: Genome biology, 巻 18, 番号 1, 9, 18.01.2017.

研究成果: ジャーナルへの寄稿コメント/討論

Sasagawa, Yohei ; Nikaido, Itoshi ; Hayashi, Tetsutaro ; Danno, Hiroki ; Uno, Kenichiro D. ; Imai, Takeshi ; Ueda, Hiroki R. / Erratum to : Quartz-Seq: A highly reproducible and sensitive single-cell RNA sequencing method, reveals non-genetic gene-expression heterogeneity. [Genome Biol. 14, (2013), (R31)]. :: Genome biology. 2017 ; 巻 18, 番号 1.
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abstract = "After publication of our article [1], we noticed some errors. In this manuscript, we amplified cDNA with 41.67 nmol/l RT primer and 70 nmol/l tagging primer for single-cell Quartz-Seq, and not pmol/l as originally stated. In addition, two mathematical expressions were inadvertently omitted. Thus, in the section Whole-transcript amplification for single-cell Quartz-Seq, the following are correct: Immediately after the second centrifugation, 0.8 μl of priming buffer (1.5× PCR buffer with MgCl2 (TaKaRa Bio), 41.67 nmol/l of the RT primer (HPLC-purified; Table 1), 4 U/μl of RNase inhibitor (RNasin Plus; Promega Corp., Madison, WI, USA), and 50 μmol/l dNTPs were added to each tube. And We then added 23 μl of the second-strand buffer (1.09× MightyAmp Buffer v2 (TaKaRa), 70 nmol/l tagging primer (HPLC-purified; Table 1), and 0.054 U/μl MightyAmp DNA polymerase (TaKaRa)) to each tube. In the section Bioinformatics analysis, the equations should appear as follows: The MI is considered the Kullback-Leibler distance from the joint probability density to the product of the marginal probability densities as follows: The MI is always non-negative, symmetric, and equal to 0 only if × and Y are independent. The MI can be represented as a summation of entropies.",
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AU - Hayashi, Tetsutaro

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AU - Imai, Takeshi

AU - Ueda, Hiroki R.

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AB - After publication of our article [1], we noticed some errors. In this manuscript, we amplified cDNA with 41.67 nmol/l RT primer and 70 nmol/l tagging primer for single-cell Quartz-Seq, and not pmol/l as originally stated. In addition, two mathematical expressions were inadvertently omitted. Thus, in the section Whole-transcript amplification for single-cell Quartz-Seq, the following are correct: Immediately after the second centrifugation, 0.8 μl of priming buffer (1.5× PCR buffer with MgCl2 (TaKaRa Bio), 41.67 nmol/l of the RT primer (HPLC-purified; Table 1), 4 U/μl of RNase inhibitor (RNasin Plus; Promega Corp., Madison, WI, USA), and 50 μmol/l dNTPs were added to each tube. And We then added 23 μl of the second-strand buffer (1.09× MightyAmp Buffer v2 (TaKaRa), 70 nmol/l tagging primer (HPLC-purified; Table 1), and 0.054 U/μl MightyAmp DNA polymerase (TaKaRa)) to each tube. In the section Bioinformatics analysis, the equations should appear as follows: The MI is considered the Kullback-Leibler distance from the joint probability density to the product of the marginal probability densities as follows: The MI is always non-negative, symmetric, and equal to 0 only if × and Y are independent. The MI can be represented as a summation of entropies.

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