TY - JOUR
T1 - Establishment and characterisation of an osteoblastic clonal cell line from human mandibular osteosarcoma (HMOS-1)
AU - Yoshikawa, H.
AU - Ohishi, M.
AU - Kohriki, S.
AU - Yoshiura, M.
AU - Ohsaki, Y.
N1 - Funding Information:
Acknowledgements--We thank Dr Y. Higuchi for the advice on the histological findings. We also thank Dr A. Yamaguchi for provision of MG-63 and HOS. This work was supported in part by Grants-in-Aid for Scientific Research 03454470 to HY from the Ministry of Education in Japan.
PY - 1997/5
Y1 - 1997/5
N2 - A human mandibular osteosarcoma cell line, HMOS-1, with osteoblastic phenotypes and tumor-genicity was established. The cell line showed high alkaline phosphatase (ALP) activity immediately after seeding, with its peak around the 7th to 10th day of culture (1.44 μmol/min/mg protein). Parathyroid hormone (PTH) enhanced the ALP activity as well as intracellular cAMP production in a concentration-dependent manner. The effects of PTH on both ALP activity and cAMP production were expressed more strongly at the end stage of logarithmic cell growth than at the resting stage. 1,25 (OH)2D3 also stimulated the ALP activity, but its effect was low and was not different in any of the different culture stages. When these cells were transplanted to BALB/C nude mice, similar tumours to the original one, with abundant osteoids were observed. However, the synthesis of type I collagen was not detected in the culture medium. The results indicate that the HMOS-1 cell line expresses an immature pre-osteoblastic phenotype. Because of these characteristics, HMOS-1 cells should be useful, not only in studies on the differentiated phenotypes of human osteoblasts, but also in studies on the diagnosis, treatment and aetiology of human osteosarcoma of the jaw.
AB - A human mandibular osteosarcoma cell line, HMOS-1, with osteoblastic phenotypes and tumor-genicity was established. The cell line showed high alkaline phosphatase (ALP) activity immediately after seeding, with its peak around the 7th to 10th day of culture (1.44 μmol/min/mg protein). Parathyroid hormone (PTH) enhanced the ALP activity as well as intracellular cAMP production in a concentration-dependent manner. The effects of PTH on both ALP activity and cAMP production were expressed more strongly at the end stage of logarithmic cell growth than at the resting stage. 1,25 (OH)2D3 also stimulated the ALP activity, but its effect was low and was not different in any of the different culture stages. When these cells were transplanted to BALB/C nude mice, similar tumours to the original one, with abundant osteoids were observed. However, the synthesis of type I collagen was not detected in the culture medium. The results indicate that the HMOS-1 cell line expresses an immature pre-osteoblastic phenotype. Because of these characteristics, HMOS-1 cells should be useful, not only in studies on the differentiated phenotypes of human osteoblasts, but also in studies on the diagnosis, treatment and aetiology of human osteosarcoma of the jaw.
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U2 - 10.1016/S0964-1955(96)00075-9
DO - 10.1016/S0964-1955(96)00075-9
M3 - Article
C2 - 9307724
AN - SCOPUS:0031147714
SN - 0964-1955
VL - 33
SP - 163
EP - 168
JO - European Journal of Cancer Part B: Oral Oncology
JF - European Journal of Cancer Part B: Oral Oncology
IS - 3
ER -
