Establishment and characterization of a cell line from Ghanaian Aedes aegypti (Diptera: Culicidae) focusing on Aedes-borne flavivirus susceptibility

Michael Amoa-Bosompem, Daisuke Kobayashi, Kentaro Itokawa, Astri Nur Faizah, Ryusei Kuwata, Samuel Dadzie, Takaya Hayashi, Shoji Yamaoka, Kyoko Sawabe, Shiroh Iwanaga, Haruhiko Isawa

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)

抄録

Mosquitoes are generally considered one of the most important vectors of arboviruses, with Aedes aegypti regarded as the most important in transmission of yellow fever and dengue viruses. To investigate why there are differences in the incidence of dengue fever and Zika in different geographical areas and an absence of outbreaks in Ghana in spite of an abundance of A. aegypti mosquitoes, we established a continuous cell line from embryonic cells of A. aegypti collected in Ghana and assessed its susceptibility to dengue, yellow fever, and Zika viruses. The new cell line (designated AeAe-GH98), having an adhesive spindle-shaped web-like morphology, was serially subcultured in both VP-12 and Schneider’s medium supplemented with 10% heat-inactivated fetal bovine serum. AeAe-GH98 cells were found to have a population doubling time of 1.3 d during exponential growth. The mosquito colony used to establish the cell line was confirmed to have originated from Africa using microsatellite assay. In terms of susceptibility to Aedes-borne flaviviruses, AeAe-GH98 cells were found to have different degrees of susceptibility to yellow fever, Zika, and dengue virus infection and propagation. While susceptibility of AeAe-GH98 cells to yellow fever and Zika viruses was comparable with that of C6/36 cells, susceptibility to dengue virus was significantly lower. This cell line will serve as a useful tool for determining molecular factors influencing virus–vector susceptibility in vitro.

本文言語英語
ページ(範囲)792-798
ページ数7
ジャーナルIn Vitro Cellular and Developmental Biology - Animal
56
9
DOI
出版ステータス出版済み - 10 2020
外部発表はい

All Science Journal Classification (ASJC) codes

  • 発生生物学
  • 細胞生物学

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