Establishment and characterization of a continuous cell line from pupal ovaries of Japanese oak silkworm Antheraea yamamai Guerin-Meneville

Shigeo Imanishi, Hajime Inoue, Takeshi Kawarabata, Koyu Hara, Masako Funakoshi, Chisa Yasunaga-Aoki, Kazuhiko Mitsuda

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

Pupal ovaries of the wild oak silkworm Antheraea yamamai Guerin-Meneville were cultured in MGM-448 (Modified Grace Medium-448) medium containing 10% fetal bovine serum. After the primary culture was set up in 1988, a continuous cell line was obtained in 1991, designated as NISES-Anya-0611 (Anya-0611). The population doubling time was 54 hrs. and 19 min. at 96 passages and 88 hrs. and 29 min. at 387 passages. Spindle-shaped and spherical cells coexisted in the cell group. The cell line karyotype line was typical of lepidopteran cell lines, consisting of numerous small chromosomes. The cell line was distinguished from other lepidopteran cell lines by comparing malic enzyme, phosphoglucose isomerase, phosphoglucose mutase, and isocitric dehydrogenase isozyme patterns. The cell line was highly infected to the Antheraea yamamai nuclear polyhedrosis virus (Anya NPV). The luciferase gene of recombinant Bm NPV (BmNPVP6ETL) was able to express in the cell line, too, so that luciferase recombinant products were able to be detected in the cell body and in supernatant. The Anya NPV clone group was isolated on the cell seat using plaque purification.

元の言語英語
ページ(範囲)1-3
ページ数3
ジャーナルIn Vitro Cellular and Developmental Biology - Animal
39
発行部数1-2
DOI
出版物ステータス出版済み - 1 1 2003

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Bombyx
Moths
Ovary
Cell Line
Nucleopolyhedrovirus
Luciferases
Intramolecular Transferases
Glucose-6-Phosphate Isomerase
Karyotype
Isoenzymes
Oxidoreductases
Clone Cells
Chromosomes
Enzymes
Serum
Population
Genes

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

これを引用

Establishment and characterization of a continuous cell line from pupal ovaries of Japanese oak silkworm Antheraea yamamai Guerin-Meneville. / Imanishi, Shigeo; Inoue, Hajime; Kawarabata, Takeshi; Hara, Koyu; Funakoshi, Masako; Yasunaga-Aoki, Chisa; Mitsuda, Kazuhiko.

:: In Vitro Cellular and Developmental Biology - Animal, 巻 39, 番号 1-2, 01.01.2003, p. 1-3.

研究成果: ジャーナルへの寄稿記事

Imanishi, Shigeo ; Inoue, Hajime ; Kawarabata, Takeshi ; Hara, Koyu ; Funakoshi, Masako ; Yasunaga-Aoki, Chisa ; Mitsuda, Kazuhiko. / Establishment and characterization of a continuous cell line from pupal ovaries of Japanese oak silkworm Antheraea yamamai Guerin-Meneville. :: In Vitro Cellular and Developmental Biology - Animal. 2003 ; 巻 39, 番号 1-2. pp. 1-3.
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