Establishment and characterization of a Fas-resistant T cell line

Masanori Higuchi, Takahiko Horiuchi, Takuya Sawabe, Shigeru Yoshizawa, Kohei Nagasawa, Yoshiyuki Niho

研究成果: ジャーナルへの寄稿記事

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Fas is a cell surface receptor that controls a signal transduction pathway leading to apoptosis. We established an antihuman Fas monoclonal antibody (mAb)-resistant variant, kit-225-FR, from the human T cell line, kit-225. Flow cytometric analysis revealed that the expression of Fas molecules on kit-225-FR was preserved. The defect in Fas molecule was not detected either by reverse transcription polymerase chain reaction (PCR) analysis of the Fas transcript or by PCR-single strand conformation polymorphism analysis of the Fas gene in kit-225-FR. Although kit-225-FR was resistant to a high concentration of anti-Fas mAb, apoptosis could be induced, as with the wild type, by exogenous C2-ceramide exposure. MORT1/FADD was expressed at wild-type level in kit-225-FR, as determined by Western blot analysis. It therefore appears that the apoptotic signal transduction in kit-225-FR is defective between FADD and the sphingomyelin-ceramide pathway. By comparing the differences from the wild-type kit-225, kit-225-FR would serve as a useful cell line for analyzing Fas-specific signal transduction pathways in detail.

元の言語英語
ページ(範囲)22-30
ページ数9
ジャーナルActa Haematologica
102
発行部数1
DOI
出版物ステータス出版済み - 9 14 1999

All Science Journal Classification (ASJC) codes

  • Hematology

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    Higuchi, M., Horiuchi, T., Sawabe, T., Yoshizawa, S., Nagasawa, K., & Niho, Y. (1999). Establishment and characterization of a Fas-resistant T cell line. Acta Haematologica, 102(1), 22-30. https://doi.org/10.1159/000040963