Establishment and characterization of a novel orthotopic mouse model for human uterine sarcoma with different metastatic potentials

Shinya Kawabe, Tetsuya Mizutani, Shin Ishikane, Miguel Ernesto Martinez, Yasushi Kiyono, Koichi Miura, Hiroshi Hosoda, Yoshitaka Imamichi, Kenji Kangawa, Kaoru Miyamoto, Yoshio Yoshida

研究成果: ジャーナルへの寄稿学術誌査読

6 被引用数 (Scopus)

抄録

Uterine sarcomas are rare and aggressive gynecologic tumors with a poor prognosis because of recurrence and metastasis. However, the mechanisms of uterine sarcoma metastasis are largely unknown. To investigate this mechanism, we developed a novel uterine sarcoma tissue-derived orthotopic and metastatic model in KSN nude mice using a green fluorescent protein stably expressed uterine sarcoma cell line, MES-SA. Histological analysis showed that all orthotopic primary tumors were undifferentiated sarcoma. Primary tumors were characterized by high 18F-fluorodeoxyglucose uptake with a positive correlation to the number of pulmonary metastases. In addition, we generated uterine sarcoma cell sublines with high or low metastatic potentials by serial in vivo selection. Microarray analysis between orthotopic tumors with high and low metastatic potentials revealed differential expression of genes related to cell proliferation and migration (TNNT1, COL1A2, and ZIC1). Our model would be useful to compensate for the limited clinical cases of uterine sarcoma and to investigate the molecular mechanisms of metastatic uterine sarcoma.

本文言語英語
ページ(範囲)182-190
ページ数9
ジャーナルCancer Letters
366
2
DOI
出版ステータス出版済み - 10月 1 2015
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 腫瘍学
  • 癌研究

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