Establishment of a new human pancreatic cancer cell line, NOR-P1, with high angiogenic activity and metastatic potential

Norihiro Sato, Kazuhiro Mizumoto, Kiichiro Beppu, Naoki Maehara, Masahiro Kusumoto, Toshinaga Nabae, Takashi Morisaki, Mitsuo Katano, Masao Tanaka

研究成果: ジャーナルへの寄稿学術誌査読

28 被引用数 (Scopus)

抄録

We present here a new cell line, NOR-P1, established from a metastatic subcutaneous tumor of a patient with pancreatic cancer. The cells show rapid growth in culture with a doubling time of 16 h and high migration activity. Genetic and molecular analyses revealed high telomerase activity and a mutation in the K-ras oncogene. Of particular interest, the cells express markedly elevated mRNA levels of angiogenic factors, vascular endothelial growth factor and platelet-derived growth factor, as well as other tumor growth-related factors. Subcutaneous transplantation of the NOR-P1 cells into nude mice formed solid, hemorrhagic tumors which were histologically diagnosed as adenocarcinoma with dense blood vessels and severe extravasation of blood. Furthermore, when NOR-P1 cell suspension was injected directly into the pancreas of nude mice, the cells grew rapidly to form intra-pancreatic tumors associated with liver metastases and peritoneal dissemination that resulted in cachexia and subsequent death. These properties suggest that NOR-P1 is an aggressive pancreatic cancer cell line with a high metastatic potential and may serve as a useful experimental model for studying tumor angiogenesis and metastasis of pancreatic cancer. Copyright (C) 2000 Elsevier Science Ireland Ltd.

本文言語英語
ページ(範囲)153-161
ページ数9
ジャーナルCancer Letters
155
2
DOI
出版ステータス出版済み - 7月 31 2000
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 腫瘍学
  • 癌研究

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