Establishment of a novel method for the production of chimeric mouse embryos using water-in-oil droplets

Hiroyuki Imai, Soichiro Tsuda, Tokuko Iwamori, Kiyoshi Kano, Ken Takeshi Kusakabe, Etsuro Ono

研究成果: Contribution to journalArticle査読

抄録

Production of chimeric animals is often a necessity for the generation of genetically modified animals and has gained popularity in recent years in regenerative medicine for the reconstruction of xenogeneic organs. Aggregation and injection methods are generally used to produce chimeric mice. In the aggregation method, the chimeras are produced by co-culturing embryos and stem cells, and keeping them physically adhered, although it may not be an assured method for producing chimeric embryos. In the injection method, the chimeras are produced by injecting stem cells into the zona pellucida using microcapillaries; however, this technique requires a high degree of skill. This study aimed to establish a novel method for producing chimeric embryos via water-in-oil droplets that differs from conventional methods. In this study, embryonic stem cells and embryos were successfully isolated in the droplets, and the emergence of chimeric embryos was confirmed by co-culture for 6 h. Using this method, the control and operability of stem cell numbers could be regulated, and reproducibility and quantification were improved during the production of chimeric embryos. In addition to the conventional methods for producing chimeric embryos, the novel method described here could be employed for the efficient production of chimeric animals. Key words:.

本文言語英語
ページ(範囲)84-90
ページ数7
ジャーナルExperimental Animals
70
1
DOI
出版ステータス出版済み - 2021

All Science Journal Classification (ASJC) codes

  • 動物科学および動物学
  • 生化学、遺伝学、分子生物学(全般)
  • 獣医学(全般)

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