Establishment of an embryonic stem (ES) cell line derived from a non-obese diabetic (NOD) mouse: In vivo differentiation into lymphocytes and potential for germ line transmission

Seiho Nagafuchi, Hitoshi Katsuta, Kazuhiko Kogawa, Tomoyuki Akashi, Shiori Kondo, Yumiko Sakai, Tadasuke Tsukiyama, Daisuke Kitamura, Yoshiyuki Niho, Takeshi Watanabe

研究成果: ジャーナルへの寄稿学術誌査読

34 被引用数 (Scopus)

抄録

A non-obese diabetic (NOD) mouse-derived embryonic stem (ES) cell line has been stably maintained in an undifferentiated state with a characteristic ES cell-like morphology, expressing the stem cell marker alkaline phosphatase, and displaying a normal diploid karyotype. After injecting the NOD-ES cells into blastocysts, chimeric mice were obtained. Small but significant numbers of lymphocytes expressed the NOD-derived MHC allele. When a chimeric mouse was mated with C57BL/6 mice, an agouti mouse was obtained, having the NOD-derived H-2 I-A(β)(g7) haplotype. Thus, an NOD-ES cell line which can differentiate into lymphocytes with potential for germ line transmission was successfully established. Copyright (C) 1999 Federation of European Biochemical Societies.

本文言語英語
ページ(範囲)101-104
ページ数4
ジャーナルFEBS Letters
455
1-2
DOI
出版ステータス出版済み - 7月 16 1999

!!!All Science Journal Classification (ASJC) codes

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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