Establishment of Caenorhabditis elegans SID-1-dependent DNA delivery system in cultured silkworm cells

Jian Xu; Kaito Yoshimura; Hiroaki Mon; Zhiqing Li; Li Zhu; Kazuhiro Iiyama; Takahiro Kusakabe; Jae Man Lee

doi:10.1007/s12033-013-9694-0

Establishment of Caenorhabditis elegans SID-1-dependent DNA delivery system in cultured silkworm cells

Jian Xu, Kaito Yoshimura, Hiroaki Mon, Zhiqing Li, Li Zhu, Kazuhiro Iiyama, Takahiro Kusakabe, Jae Man Lee

農業生物科学

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

6 被引用数 (Scopus)

抄録

The systemic RNA interference defective-1 (SID-1) can transport double-stranded RNA (dsRNA) into cytosol across the cytoplasmic membrane. We report here that ectopic expression of Caenorhabditis elegans SID-1 allows BmN4 cells to import extracellular plasmid dsDNA into cells via the direct soaking method. Interestingly, BmN4-SID1 cells incorporate dsRNA and plasmid DNA simultaneously. Furthermore, the ectopic SID-1 allows us to establish a stably transformed cell line by the simple soaking method. Our results provide an alternative method for silkworm gene function analysis with low cost and low cell toxicity.

本文言語	英語
ページ（範囲）	193-198
ページ数	6
ジャーナル	Molecular Biotechnology
巻	56
号	3
DOI	https://doi.org/10.1007/s12033-013-9694-0
出版ステータス	出版済み - 3月 2014

!!!All Science Journal Classification (ASJC) codes

バイオテクノロジー
バイオエンジニアリング
生化学
応用微生物学とバイオテクノロジー
分子生物学

文献へのアクセス

10.1007/s12033-013-9694-0

他のファイルとリンク

引用スタイル

@article{65ecb8e47f43425c89eb032856c1eec1,

title = "Establishment of Caenorhabditis elegans SID-1-dependent DNA delivery system in cultured silkworm cells",

abstract = "The systemic RNA interference defective-1 (SID-1) can transport double-stranded RNA (dsRNA) into cytosol across the cytoplasmic membrane. We report here that ectopic expression of Caenorhabditis elegans SID-1 allows BmN4 cells to import extracellular plasmid dsDNA into cells via the direct soaking method. Interestingly, BmN4-SID1 cells incorporate dsRNA and plasmid DNA simultaneously. Furthermore, the ectopic SID-1 allows us to establish a stably transformed cell line by the simple soaking method. Our results provide an alternative method for silkworm gene function analysis with low cost and low cell toxicity.",

author = "Jian Xu and Kaito Yoshimura and Hiroaki Mon and Zhiqing Li and Li Zhu and Kazuhiro Iiyama and Takahiro Kusakabe and Lee, {Jae Man}",

note = "Funding Information: Acknowledgments The cost of publication was supported in part by the Research Grant for Young Investigators of Faculty of Agriculture, Kyushu University.",

year = "2014",

month = mar,

doi = "10.1007/s12033-013-9694-0",

language = "English",

volume = "56",

pages = "193--198",

journal = "Molecular Biotechnology",

issn = "1073-6085",

publisher = "Humana Press",

number = "3",

}

TY - JOUR

T1 - Establishment of Caenorhabditis elegans SID-1-dependent DNA delivery system in cultured silkworm cells

AU - Xu, Jian

AU - Yoshimura, Kaito

AU - Mon, Hiroaki

AU - Li, Zhiqing

AU - Zhu, Li

AU - Iiyama, Kazuhiro

AU - Kusakabe, Takahiro

AU - Lee, Jae Man

N1 - Funding Information: Acknowledgments The cost of publication was supported in part by the Research Grant for Young Investigators of Faculty of Agriculture, Kyushu University.

PY - 2014/3

Y1 - 2014/3

N2 - The systemic RNA interference defective-1 (SID-1) can transport double-stranded RNA (dsRNA) into cytosol across the cytoplasmic membrane. We report here that ectopic expression of Caenorhabditis elegans SID-1 allows BmN4 cells to import extracellular plasmid dsDNA into cells via the direct soaking method. Interestingly, BmN4-SID1 cells incorporate dsRNA and plasmid DNA simultaneously. Furthermore, the ectopic SID-1 allows us to establish a stably transformed cell line by the simple soaking method. Our results provide an alternative method for silkworm gene function analysis with low cost and low cell toxicity.

AB - The systemic RNA interference defective-1 (SID-1) can transport double-stranded RNA (dsRNA) into cytosol across the cytoplasmic membrane. We report here that ectopic expression of Caenorhabditis elegans SID-1 allows BmN4 cells to import extracellular plasmid dsDNA into cells via the direct soaking method. Interestingly, BmN4-SID1 cells incorporate dsRNA and plasmid DNA simultaneously. Furthermore, the ectopic SID-1 allows us to establish a stably transformed cell line by the simple soaking method. Our results provide an alternative method for silkworm gene function analysis with low cost and low cell toxicity.

UR - http://www.scopus.com/inward/record.url?scp=84896722607&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84896722607&partnerID=8YFLogxK

U2 - 10.1007/s12033-013-9694-0

DO - 10.1007/s12033-013-9694-0

M3 - Article

C2 - 23979877

AN - SCOPUS:84896722607

SN - 1073-6085

VL - 56

SP - 193

EP - 198

JO - Molecular Biotechnology

JF - Molecular Biotechnology

IS - 3

ER -

Establishment of Caenorhabditis elegans SID-1-dependent DNA delivery system in cultured silkworm cells

抄録

!!!All Science Journal Classification (ASJC) codes

文献へのアクセス

他のファイルとリンク

フィンガープリント

引用スタイル