Esterification in organic solvents: Selection of hydrolases and effects of reaction conditions

Takuo Kawamoto, Kenji Sonomoto, Atsuo Tanaka

研究成果: Contribution to journalArticle査読

72 被引用数 (Scopus)

抄録

Fifty different hydrolases were screened for retention of high esterification activity in an organic solvent with citronellol as substrate. Although 22 hydrolases were very active as catalysts in the organic solvent, lipase from Candida cylindracea (lipase OF 360) was selected for further examination of the effects of reaction conditions on enzyme activity, with regard to catalyst availability and activity retention after immobilization. When the enzyme was entrapped in hydrophobic polyurethane gels, water-saturated isooctane was found to be the most suitable solvent, whereas polar solvents caused reversible catalyst inactivation. Entrapment significantly enhanced the operational stability of the lipase in the organic solvent.

本文言語英語
ページ(範囲)137-145
ページ数9
ジャーナルBiocatalysis and Biotransformation
1
2
DOI
出版ステータス出版済み - 1987

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 触媒
  • 生化学

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