Estimation of the freezing injury in flower buds of evergreen azaleas by water proton nuclear magnetic resonance relaxation times

Shosuke Kaku, Mari Iwaya-inoue, Lawrence V. Gusta

研究成果: ジャーナルへの寄稿記事

17 引用 (Scopus)

抄録

Temperature dependence of longitudinal relaxation times (T1) of water protons in flower buds of six azalea species differing in cold hardiness and ecological distribution was investigated by pulse nuclear magnetic resonance spectroscopy. Thermal hysteresis was observed for T1 following a slow freeze-thaw cycle. The T1 ratio (the ratio obtained from the difference between the original T1 value in an unfrozen sample and the final T1 after a freeze-thaw treatment, both at 20°C, divided by the original T1) was closely correlated with the viability of florets in non-acclimated buds of R. kiusianum. If the buds were frozen to a lethal temperature and then thawed to 20°C, the T1 ratio increased. The T1 ratios of acclimated winter buds for the six species used were correlated with the level of cold hardiness (supercooling ability of florets determined by differential thermal analysis). The T1 ratio of deacclimated spring buds, especially those from hardier species, markedly increased upon cooling to a lethal temperature. Species differences observed in acclimated winter buds disappeared upon deacclimation. The T1 ratio appears to be related to the viability of florets and the degree of freezing damage (membrane disruption) in florets.

元の言語英語
ページ(範囲)1019-1025
ページ数7
ジャーナルPlant and Cell Physiology
26
発行部数6
DOI
出版物ステータス出版済み - 1 1 1985

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Rhododendron
Freezing
protons
Protons
nuclear magnetic resonance spectroscopy
freezing
Magnetic Resonance Spectroscopy
buds
florets
Temperature
Water
Wounds and Injuries
Differential Thermal Analysis
water
cold tolerance
Hot Temperature
viability
supercooling
temperature
Membranes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

これを引用

Estimation of the freezing injury in flower buds of evergreen azaleas by water proton nuclear magnetic resonance relaxation times. / Kaku, Shosuke; Iwaya-inoue, Mari; Gusta, Lawrence V.

:: Plant and Cell Physiology, 巻 26, 番号 6, 01.01.1985, p. 1019-1025.

研究成果: ジャーナルへの寄稿記事

Kaku, Shosuke ; Iwaya-inoue, Mari ; Gusta, Lawrence V. / Estimation of the freezing injury in flower buds of evergreen azaleas by water proton nuclear magnetic resonance relaxation times. :: Plant and Cell Physiology. 1985 ; 巻 26, 番号 6. pp. 1019-1025.
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abstract = "Temperature dependence of longitudinal relaxation times (T1) of water protons in flower buds of six azalea species differing in cold hardiness and ecological distribution was investigated by pulse nuclear magnetic resonance spectroscopy. Thermal hysteresis was observed for T1 following a slow freeze-thaw cycle. The T1 ratio (the ratio obtained from the difference between the original T1 value in an unfrozen sample and the final T1 after a freeze-thaw treatment, both at 20°C, divided by the original T1) was closely correlated with the viability of florets in non-acclimated buds of R. kiusianum. If the buds were frozen to a lethal temperature and then thawed to 20°C, the T1 ratio increased. The T1 ratios of acclimated winter buds for the six species used were correlated with the level of cold hardiness (supercooling ability of florets determined by differential thermal analysis). The T1 ratio of deacclimated spring buds, especially those from hardier species, markedly increased upon cooling to a lethal temperature. Species differences observed in acclimated winter buds disappeared upon deacclimation. The T1 ratio appears to be related to the viability of florets and the degree of freezing damage (membrane disruption) in florets.",
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