Evaluation of a dysfunctional and short-lived subset of monocyte-derived dendritic cells from cancer patients

Hideya Ohnishi, Takashi Morisaki, Hideo Kuroki, Kotaro Matsumoto, Eishi Baba, Hirotaka Kuga, Masao Tanaka, Mitsuo Katano

研究成果: ジャーナルへの寄稿記事

5 引用 (Scopus)

抄録

Monocyte-derived dendritic cells (Mo-DCs) were generated from peripheral blood monocytes of 12 healthy volunteers (hMo-DCs) and 11 patients (pMo-DCs) with malignancies by culture for 7 days with granulocyte-macrophage colony-stimulating factor and interleukin-4. In this study, we focused on the cytogram pattern by FACS analysis. A gate (R1) was set up by which more than 95% of hMo-DCs were contained. Mo-DCs having lower side scatter than the R1 (R2) comprised 4.5% of hMo-DCs and 24.2% of pMo-DCs. Expressions of antigen presentation-related molecules and phagocytic ability in the R2 of pMo-DCs were lower than those in the R1 population. The R2, but not R1, in pMo-DCs decreased in number between days 7 and 14, and expression levels of antigen presentation-related molecules in the living pMo-DCs on day 14 increased. The 11 patients received dendritic cell vaccine therapy with autologous, tumor-pulsed mature Mo-DCs (day 7). The low R2 group (R2<10%, 3 patients) had a significantly higher positive delayed-type hypersensitivity reaction against autologous tumor-pulsed Mo-DCs than that of the high R2 group (R2>10%, 8 patients) (p<0.001). These results indicate that the R2 of pMo-DCs may be a dysfunctional and short-lived subset.

元の言語英語
ページ(範囲)3445-3451
ページ数7
ジャーナルAnticancer Research
25
発行部数5
出版物ステータス出版済み - 9 1 2005

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Dendritic Cells
Monocytes
Antigen Presentation
Neoplasms
Active Immunotherapy
Granulocyte-Macrophage Colony-Stimulating Factor
Cell- and Tissue-Based Therapy
Interleukin-4
Healthy Volunteers
Population

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

これを引用

Evaluation of a dysfunctional and short-lived subset of monocyte-derived dendritic cells from cancer patients. / Ohnishi, Hideya; Morisaki, Takashi; Kuroki, Hideo; Matsumoto, Kotaro; Baba, Eishi; Kuga, Hirotaka; Tanaka, Masao; Katano, Mitsuo.

:: Anticancer Research, 巻 25, 番号 5, 01.09.2005, p. 3445-3451.

研究成果: ジャーナルへの寄稿記事

Ohnishi, H, Morisaki, T, Kuroki, H, Matsumoto, K, Baba, E, Kuga, H, Tanaka, M & Katano, M 2005, 'Evaluation of a dysfunctional and short-lived subset of monocyte-derived dendritic cells from cancer patients', Anticancer Research, 巻. 25, 番号 5, pp. 3445-3451.
Ohnishi, Hideya ; Morisaki, Takashi ; Kuroki, Hideo ; Matsumoto, Kotaro ; Baba, Eishi ; Kuga, Hirotaka ; Tanaka, Masao ; Katano, Mitsuo. / Evaluation of a dysfunctional and short-lived subset of monocyte-derived dendritic cells from cancer patients. :: Anticancer Research. 2005 ; 巻 25, 番号 5. pp. 3445-3451.
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abstract = "Monocyte-derived dendritic cells (Mo-DCs) were generated from peripheral blood monocytes of 12 healthy volunteers (hMo-DCs) and 11 patients (pMo-DCs) with malignancies by culture for 7 days with granulocyte-macrophage colony-stimulating factor and interleukin-4. In this study, we focused on the cytogram pattern by FACS analysis. A gate (R1) was set up by which more than 95{\%} of hMo-DCs were contained. Mo-DCs having lower side scatter than the R1 (R2) comprised 4.5{\%} of hMo-DCs and 24.2{\%} of pMo-DCs. Expressions of antigen presentation-related molecules and phagocytic ability in the R2 of pMo-DCs were lower than those in the R1 population. The R2, but not R1, in pMo-DCs decreased in number between days 7 and 14, and expression levels of antigen presentation-related molecules in the living pMo-DCs on day 14 increased. The 11 patients received dendritic cell vaccine therapy with autologous, tumor-pulsed mature Mo-DCs (day 7). The low R2 group (R2<10{\%}, 3 patients) had a significantly higher positive delayed-type hypersensitivity reaction against autologous tumor-pulsed Mo-DCs than that of the high R2 group (R2>10{\%}, 8 patients) (p<0.001). These results indicate that the R2 of pMo-DCs may be a dysfunctional and short-lived subset.",
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AU - Ohnishi, Hideya

AU - Morisaki, Takashi

AU - Kuroki, Hideo

AU - Matsumoto, Kotaro

AU - Baba, Eishi

AU - Kuga, Hirotaka

AU - Tanaka, Masao

AU - Katano, Mitsuo

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