TY - JOUR
T1 - Evaluation of the Function of Synthetic Bimolecular Membranes and Phospholipids by Spin-Probe Method and Differential Scanning Calorimetry
AU - Honda, Shuichi
AU - Kusumoto, Naoshi
AU - Hirayama, Chuichi
AU - Kunitake, Toyoki
AU - Motozato, Yoshiaki
PY - 1983
Y1 - 1983
N2 - The amount of heat absorption of synthetic bimolecular membranes and phospholipids, which contained various polar groups and alkyl chain of different length at gel-(liquid crystal) phase transition temperature, the movements of alkyl chain part of the membranes and the differences of incorporation of probe into the membranes were studied by differential scanning calorimetry and the spin-probe method. The variation of physical properties pf the bimolecular membranes at incorporation with cholesterol into the membranes was also investigated. According to the measurements of differential scanning calorimetry, the synthetic bimolecular membranes containing ammonium salt, phosphate and sulfonate as the polar groups had larger amounts of heat absorption and transition entropy than phosphoglycerides bimolecular membrane of phosphatidylcholine type. The incorporation of 2, 2, 6, 6-tetramethyl-l-piperidinyloxyl used as a spin-probe into the phospholipids membranes was 8.7 times higher than those of the synthetic bimolecular membranes. The activation energy, obtained from the measurement of relaxation time by molecular movements using spin-probe of aliphatic acid type, was remarkably influenced by the length of alkyl chains and existence of the unsaturated bond. By the incorporation of cholesterol into the bimolecular membranes, phase transition was eliminated and the incorporation of the probe into the bimolecular membranes was disturbed. These phenomena were interpreted on the basis of a variation of fluidity of the bimolecular membranes.
AB - The amount of heat absorption of synthetic bimolecular membranes and phospholipids, which contained various polar groups and alkyl chain of different length at gel-(liquid crystal) phase transition temperature, the movements of alkyl chain part of the membranes and the differences of incorporation of probe into the membranes were studied by differential scanning calorimetry and the spin-probe method. The variation of physical properties pf the bimolecular membranes at incorporation with cholesterol into the membranes was also investigated. According to the measurements of differential scanning calorimetry, the synthetic bimolecular membranes containing ammonium salt, phosphate and sulfonate as the polar groups had larger amounts of heat absorption and transition entropy than phosphoglycerides bimolecular membrane of phosphatidylcholine type. The incorporation of 2, 2, 6, 6-tetramethyl-l-piperidinyloxyl used as a spin-probe into the phospholipids membranes was 8.7 times higher than those of the synthetic bimolecular membranes. The activation energy, obtained from the measurement of relaxation time by molecular movements using spin-probe of aliphatic acid type, was remarkably influenced by the length of alkyl chains and existence of the unsaturated bond. By the incorporation of cholesterol into the bimolecular membranes, phase transition was eliminated and the incorporation of the probe into the bimolecular membranes was disturbed. These phenomena were interpreted on the basis of a variation of fluidity of the bimolecular membranes.
UR - http://www.scopus.com/inward/record.url?scp=85010175457&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85010175457&partnerID=8YFLogxK
U2 - 10.1246/nikkashi.1983.428
DO - 10.1246/nikkashi.1983.428
M3 - Article
AN - SCOPUS:85010175457
VL - 1983
SP - 428
EP - 433
JO - Nippon Kagaku Kaishi / Chemical Society of Japan - Chemistry and Industrial Chemistry Journal
JF - Nippon Kagaku Kaishi / Chemical Society of Japan - Chemistry and Industrial Chemistry Journal
SN - 0369-4577
IS - 3
ER -