Evidence for a novel racemization process of an asparaginyl residue in mouse lysozyme under physiological conditions

K. Ueno, T. Ueda, K. Sakai, Y. Abe, N. Hamasaki, M. Okamoto, T. Imoto

研究成果: ジャーナルへの寄稿記事

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We examined chemical reactions in mouse lysozyme after incubation under physiological conditions (pH 7 and 37°C). After incubation for 8 weeks, racemization was observed specifically at Asn127 among the 19 Asp/Asn residues in mouse lysozyme. Furthermore, analysis of the primary structure showed that the racemized residue was not Asp, but Asn, which demonstrates that deamidation and isomerization did not occur. These results mean that this racemization occurs without forming a succinimide intermediate. This is the first example of D-asparaginyl formation in a protein occurring during the racemization process under physiological conditions.

元の言語英語
ページ(範囲)199-205
ページ数7
ジャーナルCellular and Molecular Life Sciences
62
発行部数2
DOI
出版物ステータス出版済み - 1 1 2005

    フィンガープリント

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Cellular and Molecular Neuroscience
  • Cell Biology

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