Evolutionary analysis of Pinus densata (Masters), a putative Tertiary hybrid. - 2. A study using species-specific chloroplast DNA markers

X. R. Wang, A. E. Szmidt

研究成果: Contribution to journalArticle査読

32 被引用数 (Scopus)

抄録

Restriction fragment analysis and heterologous hybridization of chloroplast (cp) DNA was used to develop species-specific markers for P. tabulaeformis, P. yunnanensis and P. massoniana. Fragment patterns created by the BclI and DraI restriction enzymes and hybridization patterns to the psbC and psbD probes were distinctive among the three species. No intraspecific variation was detected with respect to any of the cpDNA markers developed in this study. The cpDNA markers obtained were subsequently used to examine the parentage of P. densata, a putative Tertiary hybrid between P. tabulaeformis and P. yunnanensis. The analysis demonstrated for the first time that P. densata populations accommodate chloroplast genomes of P. tabulaeformis and P. yunnanensis, which strongly supports earlier suggestions of the hybrid origin of this species. It appears that P. densata represents a stabilized natural hybrid that has become adapted to high mountain environments where neither of the parental species can normally grow.

本文言語英語
ページ(範囲)641-647
ページ数7
ジャーナルTheoretical And Applied Genetics
80
5
DOI
出版ステータス出版済み - 1994
外部発表はい

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

フィンガープリント 「Evolutionary analysis of Pinus densata (Masters), a putative Tertiary hybrid. - 2. A study using species-specific chloroplast DNA markers」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル