Ex vivo maintenance of hematopoietic stem cells by quiescence induction through Fbxw7α overexpression

Hirono Iriuchishima, Keiyo Takubo, Sahoko Matsuoka, Ichiro Onoyama, Keiichi I. Nakayama, Yoshihisa Nojima, Toshio Suda

研究成果: ジャーナルへの寄稿記事

26 引用 (Scopus)

抄録

Cell-cycle quiescence in hematopoietic stem cells (HSCs) is essential for maintaining stemness by protecting cells from differentiation or senescence. F-box and WD-40 domain protein 7 (Fbxw7) maintains HSCs and suppresses leukemogenesis by mediating ubiquitin-dependent degradation of cell-cycle activators and oncoproteins. Fbxw7α was shown to be the preferentially expressed Fbxw7 isoform in primitive HSCs. Forced Fbxw7α expression in lineage marker Sca-1+ c-Kit+ cells led to cell-cycle dormancy by reducing the protein levels of the Fbxw7 substrates c-Myc, Notch1, and phosphorylated S6 (a key downstream element of mTOR). Hypoxia, an essential factor for HSC quiescence, suppressed c-Myc in an Fbxw7α-dependent manner. Fbxw7α-overexpressing lineage marker Sca-1+c-Kit+ cells sustained high reconstitution capacities during in vitro culture. These data suggest that Fbxw7α sustains HSC dormancy through c-Myc, Notch1, and the mTOR pathways. The modulation of Fbxw7α expression or activity represents a promising new tool for ex vivo HSC maintenance.

元の言語英語
ページ(範囲)2373-2377
ページ数5
ジャーナルBlood
117
発行部数8
DOI
出版物ステータス出版済み - 2 24 2011

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Hematopoietic Stem Cells
Stem cells
Maintenance
Cell Cycle
Cells
S 6
Cell Aging
Oncogene Proteins
Ubiquitin
Cell culture
Cell Differentiation
Protein Isoforms
Proteins
Modulation
Degradation
Substrates

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

これを引用

Ex vivo maintenance of hematopoietic stem cells by quiescence induction through Fbxw7α overexpression. / Iriuchishima, Hirono; Takubo, Keiyo; Matsuoka, Sahoko; Onoyama, Ichiro; Nakayama, Keiichi I.; Nojima, Yoshihisa; Suda, Toshio.

:: Blood, 巻 117, 番号 8, 24.02.2011, p. 2373-2377.

研究成果: ジャーナルへの寄稿記事

Iriuchishima, Hirono ; Takubo, Keiyo ; Matsuoka, Sahoko ; Onoyama, Ichiro ; Nakayama, Keiichi I. ; Nojima, Yoshihisa ; Suda, Toshio. / Ex vivo maintenance of hematopoietic stem cells by quiescence induction through Fbxw7α overexpression. :: Blood. 2011 ; 巻 117, 番号 8. pp. 2373-2377.
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