Existence of three regulatory regions each containing a highly conserved motif in the promoter of plastid-encoded RNA polymerase gene (rpoB)

Hitoshi Inada, Motoaki Seki, Hiromichi Morikawa, Mitsuo Nishimura, Koh Iba

研究成果: Contribution to journalArticle査読

12 被引用数 (Scopus)

抄録

The plastid gene rpoB encodes a plastid-specific, DNA-dependent RNA polymerase. A study of a barley mutant, albostrians, indicated that rpoB itself is transcribed by a nuclear-encoded RNA polymerase. However, the molecular nature of the nuclear-encoded RNA polymerase and mechanisms of the transcriptional regulation of rpoB expression have not been elucidated. Using an in vivo transient assay system for gene expression in plastids, the 5'-flanking region of rpoB from Arabidopsis thaliana in plastids of cultured BY-2 tobacco cells was analyzed. A series of deletion analyses of the 5'-flanking region indicated that there were two positive and one negative regulatory regions for rpoB expression. Comparison of the 5'-flanking sequences of rpoB among several plant species revealed the existence of three conserved motifs in these regions.

本文言語英語
ページ(範囲)883-890
ページ数8
ジャーナルPlant Journal
11
4
DOI
出版ステータス出版済み - 4 1997

All Science Journal Classification (ASJC) codes

  • 遺伝学
  • 植物科学
  • 細胞生物学

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