Exosomes secreted from monocyte-derived dendritic cells support in vitro naive CD4+ T cell survival through NF-κB activation

Kotaro Matsumoto, Takashi Morisaki, Hideo Kuroki, Makoto Kubo, Hideya Ohnishi, Katsuya Nakamura, Chihiro Nakahara, Hirotaka Kuga, Eishi Baba, Masafumi Nakamura, Kazuho Hirata, Masao Tanaka, Mitsuo Katano

研究成果: ジャーナルへの寄稿記事

16 引用 (Scopus)

抄録

We investigated the effect of exosomes secreted from human monocyte-derived dendritic cells (Mo-DCs), which are generated from PBMCs in response to treatment with GM-CSF and IL-4, on naive CD4+ T cell survival in vitro. Exosomes isolated from culture supernatants of Mo-DCs (>90% purity) were purified with anti-HLA-DP, -DQ, -DR-coated paramagnetic beads. Purified exosomes prolonged the survival of naive CD4+ T cells (>98% purity) in vitro. Treatment with neutralizing mAb against HLA-DR significantly decreased the supportive effect of purified exosomes on CD4+ T cell survival. Exosomes increased nuclear translocation of NF-κB in naive CD4+ T cells, and NF-κB activation was significantly suppressed by anti-HLA-DR mAb or NF-κB inhibitor pyrrolidine dithiocarbamate (PDTC). In addition, PDTC inhibited the effect of exosomes on naive CD4+ T cell survival. Thus, exosomes secreted by Mo-DCs appear to support naive CD4+ T cell survival via NF-κB activation induced by interaction of HLA-DR and TCRs.

元の言語英語
ページ(範囲)20-29
ページ数10
ジャーナルCellular Immunology
231
発行部数1-2
DOI
出版物ステータス出版済み - 9 1 2004

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Exosomes
Dendritic Cells
Monocytes
Cell Survival
T-Lymphocytes
HLA-DR Antigens
HLA-DP Antigens
HLA-DQ Antigens
Granulocyte-Macrophage Colony-Stimulating Factor
In Vitro Techniques
Interleukin-4
Survival

All Science Journal Classification (ASJC) codes

  • Immunology

これを引用

Exosomes secreted from monocyte-derived dendritic cells support in vitro naive CD4+ T cell survival through NF-κB activation. / Matsumoto, Kotaro; Morisaki, Takashi; Kuroki, Hideo; Kubo, Makoto; Ohnishi, Hideya; Nakamura, Katsuya; Nakahara, Chihiro; Kuga, Hirotaka; Baba, Eishi; Nakamura, Masafumi; Hirata, Kazuho; Tanaka, Masao; Katano, Mitsuo.

:: Cellular Immunology, 巻 231, 番号 1-2, 01.09.2004, p. 20-29.

研究成果: ジャーナルへの寄稿記事

Matsumoto, Kotaro ; Morisaki, Takashi ; Kuroki, Hideo ; Kubo, Makoto ; Ohnishi, Hideya ; Nakamura, Katsuya ; Nakahara, Chihiro ; Kuga, Hirotaka ; Baba, Eishi ; Nakamura, Masafumi ; Hirata, Kazuho ; Tanaka, Masao ; Katano, Mitsuo. / Exosomes secreted from monocyte-derived dendritic cells support in vitro naive CD4+ T cell survival through NF-κB activation. :: Cellular Immunology. 2004 ; 巻 231, 番号 1-2. pp. 20-29.
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AU - Ohnishi, Hideya

AU - Nakamura, Katsuya

AU - Nakahara, Chihiro

AU - Kuga, Hirotaka

AU - Baba, Eishi

AU - Nakamura, Masafumi

AU - Hirata, Kazuho

AU - Tanaka, Masao

AU - Katano, Mitsuo

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