Exploration of bacterial species associated with the salivary microbiome of individuals with a low susceptibility to dental caries

Haruna Yasunaga, Toru Takeshita, Yukie Shibata, Michiko Furuta, Yoshihiro Shimazaki, Sumio Akifusa, Toshiharu Ninomiya, Yutaka Kiyohara, Ichiro Takahashi, Yoshihisa Yamashita

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

Objective: Dental caries is caused by acidogenic plaque microbiota formed on saliva-bathed tooth surfaces, in which multiple organisms act collectively to initiate and expand a cavity. We explored bacterial species associated with the salivary microbiome of individuals with low susceptibility to dental caries. Materials and methods: The bacterial composition of saliva from 19 young adults was analyzed using barcoded pyrosequencing of the 16S rRNA gene; we compared 10 caries-experienced (CE) and nine caries-free (CF) individuals. A quantitative PCR assay of saliva from 139 orally healthy adults aged 40–59 years was carried out to confirm the result obtained by pyrosequencing analysis. Results: The microbiomes of CF individuals showed more diverse communities with a significantly greater proportion of the genus Porphyromonas. Among operational taxonomic units (OTUs) corresponding to the genus Porphyromonas, the OTU corresponding to P. pasteri was the most predominant and its relative abundance in CF individuals was significantly greater than in CE individuals (P < 0.001, Wilcoxon rank sum test). A quantitative PCR assay of saliva confirmed that the amounts of P. pasteri were significantly higher in individuals with lower caries experience (filled teeth <15, n = 67) than in those with higher caries experience (filled teeth ≥15, n = 72) (P < 0.001, Student’s t test). Conclusion: These results revealed an association between a greater abundance of P. pasteri and lower susceptibility to dental caries. Clinical relevance: P. pasteri may be a bacterial species that could potentially be used as a marker for maintaining a healthy oral microbiome against dental caries.

元の言語英語
ページ(範囲)2399-2406
ページ数8
ジャーナルClinical Oral Investigations
21
発行部数8
DOI
出版物ステータス出版済み - 11 1 2017

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Dental Caries Susceptibility
Microbiota
Porphyromonas
Saliva
Tooth
Dental Caries
Nonparametric Statistics
Polymerase Chain Reaction
rRNA Genes
Young Adult
Students

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

これを引用

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title = "Exploration of bacterial species associated with the salivary microbiome of individuals with a low susceptibility to dental caries",
abstract = "Objective: Dental caries is caused by acidogenic plaque microbiota formed on saliva-bathed tooth surfaces, in which multiple organisms act collectively to initiate and expand a cavity. We explored bacterial species associated with the salivary microbiome of individuals with low susceptibility to dental caries. Materials and methods: The bacterial composition of saliva from 19 young adults was analyzed using barcoded pyrosequencing of the 16S rRNA gene; we compared 10 caries-experienced (CE) and nine caries-free (CF) individuals. A quantitative PCR assay of saliva from 139 orally healthy adults aged 40–59 years was carried out to confirm the result obtained by pyrosequencing analysis. Results: The microbiomes of CF individuals showed more diverse communities with a significantly greater proportion of the genus Porphyromonas. Among operational taxonomic units (OTUs) corresponding to the genus Porphyromonas, the OTU corresponding to P. pasteri was the most predominant and its relative abundance in CF individuals was significantly greater than in CE individuals (P < 0.001, Wilcoxon rank sum test). A quantitative PCR assay of saliva confirmed that the amounts of P. pasteri were significantly higher in individuals with lower caries experience (filled teeth <15, n = 67) than in those with higher caries experience (filled teeth ≥15, n = 72) (P < 0.001, Student’s t test). Conclusion: These results revealed an association between a greater abundance of P. pasteri and lower susceptibility to dental caries. Clinical relevance: P. pasteri may be a bacterial species that could potentially be used as a marker for maintaining a healthy oral microbiome against dental caries.",
author = "Haruna Yasunaga and Toru Takeshita and Yukie Shibata and Michiko Furuta and Yoshihiro Shimazaki and Sumio Akifusa and Toshiharu Ninomiya and Yutaka Kiyohara and Ichiro Takahashi and Yoshihisa Yamashita",
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T1 - Exploration of bacterial species associated with the salivary microbiome of individuals with a low susceptibility to dental caries

AU - Yasunaga, Haruna

AU - Takeshita, Toru

AU - Shibata, Yukie

AU - Furuta, Michiko

AU - Shimazaki, Yoshihiro

AU - Akifusa, Sumio

AU - Ninomiya, Toshiharu

AU - Kiyohara, Yutaka

AU - Takahashi, Ichiro

AU - Yamashita, Yoshihisa

PY - 2017/11/1

Y1 - 2017/11/1

N2 - Objective: Dental caries is caused by acidogenic plaque microbiota formed on saliva-bathed tooth surfaces, in which multiple organisms act collectively to initiate and expand a cavity. We explored bacterial species associated with the salivary microbiome of individuals with low susceptibility to dental caries. Materials and methods: The bacterial composition of saliva from 19 young adults was analyzed using barcoded pyrosequencing of the 16S rRNA gene; we compared 10 caries-experienced (CE) and nine caries-free (CF) individuals. A quantitative PCR assay of saliva from 139 orally healthy adults aged 40–59 years was carried out to confirm the result obtained by pyrosequencing analysis. Results: The microbiomes of CF individuals showed more diverse communities with a significantly greater proportion of the genus Porphyromonas. Among operational taxonomic units (OTUs) corresponding to the genus Porphyromonas, the OTU corresponding to P. pasteri was the most predominant and its relative abundance in CF individuals was significantly greater than in CE individuals (P < 0.001, Wilcoxon rank sum test). A quantitative PCR assay of saliva confirmed that the amounts of P. pasteri were significantly higher in individuals with lower caries experience (filled teeth <15, n = 67) than in those with higher caries experience (filled teeth ≥15, n = 72) (P < 0.001, Student’s t test). Conclusion: These results revealed an association between a greater abundance of P. pasteri and lower susceptibility to dental caries. Clinical relevance: P. pasteri may be a bacterial species that could potentially be used as a marker for maintaining a healthy oral microbiome against dental caries.

AB - Objective: Dental caries is caused by acidogenic plaque microbiota formed on saliva-bathed tooth surfaces, in which multiple organisms act collectively to initiate and expand a cavity. We explored bacterial species associated with the salivary microbiome of individuals with low susceptibility to dental caries. Materials and methods: The bacterial composition of saliva from 19 young adults was analyzed using barcoded pyrosequencing of the 16S rRNA gene; we compared 10 caries-experienced (CE) and nine caries-free (CF) individuals. A quantitative PCR assay of saliva from 139 orally healthy adults aged 40–59 years was carried out to confirm the result obtained by pyrosequencing analysis. Results: The microbiomes of CF individuals showed more diverse communities with a significantly greater proportion of the genus Porphyromonas. Among operational taxonomic units (OTUs) corresponding to the genus Porphyromonas, the OTU corresponding to P. pasteri was the most predominant and its relative abundance in CF individuals was significantly greater than in CE individuals (P < 0.001, Wilcoxon rank sum test). A quantitative PCR assay of saliva confirmed that the amounts of P. pasteri were significantly higher in individuals with lower caries experience (filled teeth <15, n = 67) than in those with higher caries experience (filled teeth ≥15, n = 72) (P < 0.001, Student’s t test). Conclusion: These results revealed an association between a greater abundance of P. pasteri and lower susceptibility to dental caries. Clinical relevance: P. pasteri may be a bacterial species that could potentially be used as a marker for maintaining a healthy oral microbiome against dental caries.

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U2 - 10.1007/s00784-016-2035-5

DO - 10.1007/s00784-016-2035-5

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JO - Clinical Oral Investigations

JF - Clinical Oral Investigations

SN - 1432-6981

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