Expression analysis of cytochrome P450s in the silkmoth, Bombyx mori

Kohji Yamamoto, Hirofumi Ichinose, Yoichi Aso, Hiroshi Fujii

研究成果: ジャーナルへの寄稿記事

15 引用 (Scopus)

抄録

Cytochrome P450 (CYP) is a superfamily of proteins involved in a variety of physiological phenomena. Four cDNAs encoding silkmoth cytochrome P450 proteins, cyp4M5, cyp6AB4, cyp9A20 and cyp9A22, were cloned by reverse transcriptase-polymerase chain reaction. These cDNAs showed identities of 21-24% to each other at the amino acid level. Expression analysis showed that CYP mRNAs were expressed in a tissue-specific manner and that expression was developmentally regulated in the fat body and midgut. We found that various cyps were upregulated in the fat body after exposure to organophosphorus, neonicotinoid and pyrethroid insecticides. Our results suggest that over-expressed CYPs can contribute to insecticide resistance in Lepidopterans.

元の言語英語
ページ(範囲)1-6
ページ数6
ジャーナルPesticide Biochemistry and Physiology
97
発行部数1
DOI
出版物ステータス出版済み - 5 1 2010

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Bombyx
Cytochromes
Bombyx mori
cytochromes
cytochrome P-450
Cytochrome P-450 Enzyme System
Fat Body
fat body
Complementary DNA
Insecticide Resistance
Physiological Phenomena
Pyrethrins
neonicotinoid insecticides
pyrethroid insecticides
organophosphorus insecticides
insecticide resistance
Insecticides
Reverse Transcriptase Polymerase Chain Reaction
midgut
Proteins

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Health, Toxicology and Mutagenesis

これを引用

Expression analysis of cytochrome P450s in the silkmoth, Bombyx mori. / Yamamoto, Kohji; Ichinose, Hirofumi; Aso, Yoichi; Fujii, Hiroshi.

:: Pesticide Biochemistry and Physiology, 巻 97, 番号 1, 01.05.2010, p. 1-6.

研究成果: ジャーナルへの寄稿記事

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