Expression analysis of Escherichia coli lacZ reporter gene in transgenic mice

Eiki Takahashi, Norimasa Miyamoto, Noriko Kajiwara, Keiko Furuya, Keiko Yanai-Taniguchi, Fumihiro Sugiyama, Ken Ichi Yagami

研究成果: Contribution to journalArticle査読

6 被引用数 (Scopus)

抄録

To define a gene expression mechanism, it is often advantageous to use a reporter gene and transgenic mouse. The lacZ reporter gene is particularly useful for studies of the cis-regulatory element for tissue-specific expression in transgenic mice because of the ease of the enzyme assay and visualization on sections. In this report, we describe our method for examining the cis-regulatory element in transgenic mice, including choice of the lacZ gene, generation of transgenic mice, and analysis of β-galactosidase activity. Themes: Cellular and molecular biology. Topics: Staining, tracing, and imaging techniques. Copyright (C) 2000 Elsevier Science B.V.

本文言語英語
ページ(範囲)159-166
ページ数8
ジャーナルBrain Research Protocols
5
2
DOI
出版ステータス出版済み - 4 2000
外部発表はい

All Science Journal Classification (ASJC) codes

  • 神経科学(全般)

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