Expression analysis of sex-specific and 17β-estradiol-responsive genes in the Japanese medaka, Oryzias latipes, using oligonucleotide microarrays

Katsuyuki Kishi, Emiko Kitagawa, Norio Onikura, Akiko Nakamura, Hitoshi Iwahashi

研究成果: Contribution to journalArticle

34 引用 (Scopus)

抜粋

Gene profiling of Japanese medaka (Oryzias latipes) was performed using an oligonucleotide DNA microarray representing 22,587 TIGR O. latipes gene indices (OLGIs). The average correlation coefficients for gene expression between individual mature fish were high (>0.95) for both female and male, indicating that the physiological status of medaka is highly reproducible under prescribed growth conditions. Of the 22,587 OLGIs, 2575 showed significant differences in expression between female and male. Exposure to 17β-estradiol (E2) revealed 381 E2-responsive OLGIs in male medaka. Feminization and male-dysfunction factors of the E2-treated males calculated using the combination of Pearson correlation coefficient and Euclidean distances indicate that E2 treatment "weakly feminized" male medaka, while male physiological functions were not significantly disrupted. This study demonstrates the possibility of using medaka microarrays to estimate the overall effects of hormonally active chemicals.

元の言語英語
ページ(範囲)241-251
ページ数11
ジャーナルGenomics
88
発行部数2
DOI
出版物ステータス出版済み - 8 1 2006
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Genetics

フィンガープリント Expression analysis of sex-specific and 17β-estradiol-responsive genes in the Japanese medaka, Oryzias latipes, using oligonucleotide microarrays' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用