Expression and effects of glial cell line-derived neurotrophic factor on periodontal ligament cells

Naohide Yamamoto, Hidefumi Maeda, Atsushi Tomokiyo, Shinsuke Fujii, Naohisa Wada, Satoshi Monnouchi, Kiyomi Kono, Katsuaki Koori, Yoko Teramatsu, Akifumi Akamine

研究成果: ジャーナルへの寄稿記事

9 引用 (Scopus)

抄録

Aim To investigate Glial cell line-derived neurotrophic factor (GDNF) expression in normal and wounded rat periodontal ligament (PDL) and the effects of GDNF on human PDL cells (HPDLCs) migration and extracellular matrix expression in HPDLCs. Material and Methods The expression of GDNF and GDNF receptors was examined by immunocyto/histochemical analyses. Gene expression in HPDLCs treated with GDNF, interleukin-1 beta (IL-1β), or tumour necrosis factor-alpha (TNF-α) was quantified by quantitative RT-PCR (qRT-PCR). In addition, we examined the migratory effect of GDNF on HPDLCs. Results GDNF was expressed in normal rat PDL and cultured HPDLCs. HPDLCs also expressed GDNF receptors. In wounded rat PDL, GDNF expression was up-regulated. QRT-PCR analysis revealed that IL-1β and TNF-α significantly increased the expression of GDNF in HPDLCs. Furthermore, GDNF induced migration of HPDLCs, which was blocked by pre-treatment with the peptide including Arg-Gly-Asp (RGD) sequence, or neutralizing antibodies against integrin αVβ3 or GDNF. Also, GDNF up-regulated expression of bone sialoprotein (BSP) and fibronectin in HPDLCs. Conclusions GDNF expression is increased in rat wounded PDL tissue and HPDLCs treated with pro-inflammatory cytokines. GDNF enhances the expression of BSP and fibronectin, and migration in an RGD-dependent manner via the integrin αVβ3. These findings suggest that GDNF may contribute to wound healing in PDL tissue.

元の言語英語
ページ(範囲)556-564
ページ数9
ジャーナルJournal of Clinical Periodontology
39
発行部数6
DOI
出版物ステータス出版済み - 6 2012

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Glial Cell Line-Derived Neurotrophic Factor
Periodontal Ligament
Glial Cell Line-Derived Neurotrophic Factor Receptors
Integrin-Binding Sialoprotein
Interleukin-1beta
Fibronectins
Integrins
Tumor Necrosis Factor-alpha
Polymerase Chain Reaction
Neutralizing Antibodies
Wound Healing

All Science Journal Classification (ASJC) codes

  • Periodontics

これを引用

Expression and effects of glial cell line-derived neurotrophic factor on periodontal ligament cells. / Yamamoto, Naohide; Maeda, Hidefumi; Tomokiyo, Atsushi; Fujii, Shinsuke; Wada, Naohisa; Monnouchi, Satoshi; Kono, Kiyomi; Koori, Katsuaki; Teramatsu, Yoko; Akamine, Akifumi.

:: Journal of Clinical Periodontology, 巻 39, 番号 6, 06.2012, p. 556-564.

研究成果: ジャーナルへの寄稿記事

Yamamoto, Naohide ; Maeda, Hidefumi ; Tomokiyo, Atsushi ; Fujii, Shinsuke ; Wada, Naohisa ; Monnouchi, Satoshi ; Kono, Kiyomi ; Koori, Katsuaki ; Teramatsu, Yoko ; Akamine, Akifumi. / Expression and effects of glial cell line-derived neurotrophic factor on periodontal ligament cells. :: Journal of Clinical Periodontology. 2012 ; 巻 39, 番号 6. pp. 556-564.
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abstract = "Aim To investigate Glial cell line-derived neurotrophic factor (GDNF) expression in normal and wounded rat periodontal ligament (PDL) and the effects of GDNF on human PDL cells (HPDLCs) migration and extracellular matrix expression in HPDLCs. Material and Methods The expression of GDNF and GDNF receptors was examined by immunocyto/histochemical analyses. Gene expression in HPDLCs treated with GDNF, interleukin-1 beta (IL-1β), or tumour necrosis factor-alpha (TNF-α) was quantified by quantitative RT-PCR (qRT-PCR). In addition, we examined the migratory effect of GDNF on HPDLCs. Results GDNF was expressed in normal rat PDL and cultured HPDLCs. HPDLCs also expressed GDNF receptors. In wounded rat PDL, GDNF expression was up-regulated. QRT-PCR analysis revealed that IL-1β and TNF-α significantly increased the expression of GDNF in HPDLCs. Furthermore, GDNF induced migration of HPDLCs, which was blocked by pre-treatment with the peptide including Arg-Gly-Asp (RGD) sequence, or neutralizing antibodies against integrin αVβ3 or GDNF. Also, GDNF up-regulated expression of bone sialoprotein (BSP) and fibronectin in HPDLCs. Conclusions GDNF expression is increased in rat wounded PDL tissue and HPDLCs treated with pro-inflammatory cytokines. GDNF enhances the expression of BSP and fibronectin, and migration in an RGD-dependent manner via the integrin αVβ3. These findings suggest that GDNF may contribute to wound healing in PDL tissue.",
author = "Naohide Yamamoto and Hidefumi Maeda and Atsushi Tomokiyo and Shinsuke Fujii and Naohisa Wada and Satoshi Monnouchi and Kiyomi Kono and Katsuaki Koori and Yoko Teramatsu and Akifumi Akamine",
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T1 - Expression and effects of glial cell line-derived neurotrophic factor on periodontal ligament cells

AU - Yamamoto, Naohide

AU - Maeda, Hidefumi

AU - Tomokiyo, Atsushi

AU - Fujii, Shinsuke

AU - Wada, Naohisa

AU - Monnouchi, Satoshi

AU - Kono, Kiyomi

AU - Koori, Katsuaki

AU - Teramatsu, Yoko

AU - Akamine, Akifumi

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N2 - Aim To investigate Glial cell line-derived neurotrophic factor (GDNF) expression in normal and wounded rat periodontal ligament (PDL) and the effects of GDNF on human PDL cells (HPDLCs) migration and extracellular matrix expression in HPDLCs. Material and Methods The expression of GDNF and GDNF receptors was examined by immunocyto/histochemical analyses. Gene expression in HPDLCs treated with GDNF, interleukin-1 beta (IL-1β), or tumour necrosis factor-alpha (TNF-α) was quantified by quantitative RT-PCR (qRT-PCR). In addition, we examined the migratory effect of GDNF on HPDLCs. Results GDNF was expressed in normal rat PDL and cultured HPDLCs. HPDLCs also expressed GDNF receptors. In wounded rat PDL, GDNF expression was up-regulated. QRT-PCR analysis revealed that IL-1β and TNF-α significantly increased the expression of GDNF in HPDLCs. Furthermore, GDNF induced migration of HPDLCs, which was blocked by pre-treatment with the peptide including Arg-Gly-Asp (RGD) sequence, or neutralizing antibodies against integrin αVβ3 or GDNF. Also, GDNF up-regulated expression of bone sialoprotein (BSP) and fibronectin in HPDLCs. Conclusions GDNF expression is increased in rat wounded PDL tissue and HPDLCs treated with pro-inflammatory cytokines. GDNF enhances the expression of BSP and fibronectin, and migration in an RGD-dependent manner via the integrin αVβ3. These findings suggest that GDNF may contribute to wound healing in PDL tissue.

AB - Aim To investigate Glial cell line-derived neurotrophic factor (GDNF) expression in normal and wounded rat periodontal ligament (PDL) and the effects of GDNF on human PDL cells (HPDLCs) migration and extracellular matrix expression in HPDLCs. Material and Methods The expression of GDNF and GDNF receptors was examined by immunocyto/histochemical analyses. Gene expression in HPDLCs treated with GDNF, interleukin-1 beta (IL-1β), or tumour necrosis factor-alpha (TNF-α) was quantified by quantitative RT-PCR (qRT-PCR). In addition, we examined the migratory effect of GDNF on HPDLCs. Results GDNF was expressed in normal rat PDL and cultured HPDLCs. HPDLCs also expressed GDNF receptors. In wounded rat PDL, GDNF expression was up-regulated. QRT-PCR analysis revealed that IL-1β and TNF-α significantly increased the expression of GDNF in HPDLCs. Furthermore, GDNF induced migration of HPDLCs, which was blocked by pre-treatment with the peptide including Arg-Gly-Asp (RGD) sequence, or neutralizing antibodies against integrin αVβ3 or GDNF. Also, GDNF up-regulated expression of bone sialoprotein (BSP) and fibronectin in HPDLCs. Conclusions GDNF expression is increased in rat wounded PDL tissue and HPDLCs treated with pro-inflammatory cytokines. GDNF enhances the expression of BSP and fibronectin, and migration in an RGD-dependent manner via the integrin αVβ3. These findings suggest that GDNF may contribute to wound healing in PDL tissue.

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