Expression and functional analyses of the Dxpa gene, the Drosophila homolog of the human excision repair gene XPA

T. Shimamoto, Teiichi Tanimura, Y. Yoneda, Y. Kobayakawa, K. Sugasawa, F. Hanaoka, M. Oka, Y. Okada, K. Tanaka, K. Kohno

研究成果: ジャーナルへの寄稿記事

17 引用 (Scopus)

抄録

Xeroderma pigmentosum (XP) is a human hereditary disease characterized by a defect in DNA repair after exposure to ultraviolet light. Among the seven groups of XP, group A (XP-A) patients show the most severe deficiency in excision repair and a wide variety of cutaneous and neurological disorders. We have cloned homologs of the human XPA gene from chicken, Xenopus, and Drosophila, and sequence analysis revealed that these genes are highly conserved throughout evolution. Here, we report characterization of the Drosophila homolog of the human XPA gene (Dxpa). The Dxpa gene product shows DNA repair activities in an in vitro repair system, and Dxpa cDNA has been shown to complement a mutant allele of human XP-A cells by transfection. Polytene chromosome in situ hybridization mapped Dxpa to 3F6-8 on the X chromosome, where no mutant defective in excision repair was reported. Northern blot analysis showed that the gene is continuously expressed in all stages of fly development. Interestingly, the Dxpa protein is strongly expressed in the central nervous system and muscles as revealed by immunohistochemical analysis using anti-Dxpa antibodies, consistent with the results obtained in transgenic flies expressing a Dxpa-β-galactosidase fusion gene driven by the Dxpa promoter.

元の言語英語
ページ(範囲)22452-22459
ページ数8
ジャーナルJournal of Biological Chemistry
270
発行部数38
DOI
出版物ステータス出版済み - 1 1 1995

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DNA Repair
Drosophila
Repair
Xeroderma Pigmentosum
Genes
Diptera
Chromosomes
Galactosidases
Polytene Chromosomes
Inborn Genetic Diseases
Gene Fusion
X Chromosome
Ultraviolet Rays
Xenopus
Nervous System Diseases
Northern Blotting
DNA
Neurology
In Situ Hybridization
Transfection

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Expression and functional analyses of the Dxpa gene, the Drosophila homolog of the human excision repair gene XPA. / Shimamoto, T.; Tanimura, Teiichi; Yoneda, Y.; Kobayakawa, Y.; Sugasawa, K.; Hanaoka, F.; Oka, M.; Okada, Y.; Tanaka, K.; Kohno, K.

:: Journal of Biological Chemistry, 巻 270, 番号 38, 01.01.1995, p. 22452-22459.

研究成果: ジャーナルへの寄稿記事

Shimamoto, T, Tanimura, T, Yoneda, Y, Kobayakawa, Y, Sugasawa, K, Hanaoka, F, Oka, M, Okada, Y, Tanaka, K & Kohno, K 1995, 'Expression and functional analyses of the Dxpa gene, the Drosophila homolog of the human excision repair gene XPA', Journal of Biological Chemistry, 巻. 270, 番号 38, pp. 22452-22459. https://doi.org/10.1074/jbc.270.38.22452
Shimamoto, T. ; Tanimura, Teiichi ; Yoneda, Y. ; Kobayakawa, Y. ; Sugasawa, K. ; Hanaoka, F. ; Oka, M. ; Okada, Y. ; Tanaka, K. ; Kohno, K. / Expression and functional analyses of the Dxpa gene, the Drosophila homolog of the human excision repair gene XPA. :: Journal of Biological Chemistry. 1995 ; 巻 270, 番号 38. pp. 22452-22459.
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abstract = "Xeroderma pigmentosum (XP) is a human hereditary disease characterized by a defect in DNA repair after exposure to ultraviolet light. Among the seven groups of XP, group A (XP-A) patients show the most severe deficiency in excision repair and a wide variety of cutaneous and neurological disorders. We have cloned homologs of the human XPA gene from chicken, Xenopus, and Drosophila, and sequence analysis revealed that these genes are highly conserved throughout evolution. Here, we report characterization of the Drosophila homolog of the human XPA gene (Dxpa). The Dxpa gene product shows DNA repair activities in an in vitro repair system, and Dxpa cDNA has been shown to complement a mutant allele of human XP-A cells by transfection. Polytene chromosome in situ hybridization mapped Dxpa to 3F6-8 on the X chromosome, where no mutant defective in excision repair was reported. Northern blot analysis showed that the gene is continuously expressed in all stages of fly development. Interestingly, the Dxpa protein is strongly expressed in the central nervous system and muscles as revealed by immunohistochemical analysis using anti-Dxpa antibodies, consistent with the results obtained in transgenic flies expressing a Dxpa-β-galactosidase fusion gene driven by the Dxpa promoter.",
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