Expression and purification of uniformly 15N-labeled amyloid β peptide 1-40 in Escherichia coli

M. Nagata-Uchiyama; M. Yaguchi; Y. Hirano; Tadashi Ueda

doi:10.2174/092986607781483741

Expression and purification of uniformly ¹⁵N-labeled amyloid β peptide 1-40 in Escherichia coli

M. Nagata-Uchiyama, M. Yaguchi, Y. Hirano, Tadashi Ueda

生命薬学

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

14 被引用数 (Scopus)

抄録

For biophysical studies using heteronuclear NMR analysis of amyloid beta peptide, construction of an efficient and high yield expression system of uniformly isotopic labeled amyloid beta peptide is desirable. Here we succeeded in obtaining ¹⁵N-labeled amyloid beta 1-40 expressed by attachment to hen egg white lysozyme as a fusion protein.

本文言語	英語
ページ（範囲）	788-792
ページ数	5
ジャーナル	Protein and Peptide Letters
巻	14
号	8
DOI	https://doi.org/10.2174/092986607781483741
出版ステータス	出版済み - 8月 1 2007

!!!All Science Journal Classification (ASJC) codes

構造生物学
生化学

文献へのアクセス

10.2174/092986607781483741

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引用スタイル

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