Expression dynamics of bovine MX genes in the endometrium and placenta during early to mid pregnancy

Takahiro Shirozu, Keisuke Sasaki, Manabu Kawahara, Yojiro Yanagawa, Masashi Nagano, Nobuhiko Yamauchi, Masashi Takahashi

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

MX belongs to a family of type I interferon (IFN)-stimulated genes, and the MX protein has antiviral activity. MX has at least two isoforms, known as MX1 and MX2, in mammals. Moreover, bovine MX1 has been found to have alternative splice variants—namely, MX1-a and MX1B. In ruminants, IFN-τ—a type I IFN—is temporarily produced from the conceptus before implantation and induces MX expression in the endometrium. However, the expression dynamics of MX after implantation are not clear. In the present study, we investigated the expression of MX1-a, MX1B and MX2 in the endometrium and placenta before and after implantation along with the expression of IFN-α, type I receptors (IFNAR1 and IFNAR2) and interferon regulatory factors (IRF3 and IRF9). Pregnant uterine samples were divided into five groups according to pregnancy days 14–18, 25–40, 50–70, 80–100, and 130–150. Tissue samples were collected from the intercaruncular endometrium (IC), caruncular endometrium (C) and fetal placenta (P). Although all the MX expressions were significantly higher in the IC and C at days 14–18, presumably caused by embryo-secreted IFN-τ stimulation, their expressions were also detectable in the IC, C and P after implantation. Furthermore, IFN-α expression was significantly higher in the IC. RT-PCR indicated IFNAR1, IFNAR2, IRF3 and IRF9 mRNA in all the tissues during pregnancy. These results suggest that all the MX genes are affected by the type I IFN pathway during pregnancy and are involved in an immune response to protect the mother and fetus.

元の言語英語
ページ(範囲)29-35
ページ数7
ジャーナルJournal of Reproduction and Development
62
発行部数1
DOI
出版物ステータス出版済み - 2 20 2016

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endometrium
placenta
interferons
pregnancy
cattle
genes
antiviral proteins
conceptus
fetus
ruminants
embryo (animal)
reverse transcriptase polymerase chain reaction
immune response
mammals
sampling
receptors

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

これを引用

Expression dynamics of bovine MX genes in the endometrium and placenta during early to mid pregnancy. / Shirozu, Takahiro; Sasaki, Keisuke; Kawahara, Manabu; Yanagawa, Yojiro; Nagano, Masashi; Yamauchi, Nobuhiko; Takahashi, Masashi.

:: Journal of Reproduction and Development, 巻 62, 番号 1, 20.02.2016, p. 29-35.

研究成果: ジャーナルへの寄稿記事

Shirozu, Takahiro ; Sasaki, Keisuke ; Kawahara, Manabu ; Yanagawa, Yojiro ; Nagano, Masashi ; Yamauchi, Nobuhiko ; Takahashi, Masashi. / Expression dynamics of bovine MX genes in the endometrium and placenta during early to mid pregnancy. :: Journal of Reproduction and Development. 2016 ; 巻 62, 番号 1. pp. 29-35.
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