Expression of a functional sphingomyelinase of Pseudomonas sp. TK4 in mammalian cells

Yasuhiro Horibata, Noriyuki Sueyoshi, Makoto Ito

研究成果: ジャーナルへの寄稿記事

5 引用 (Scopus)

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We report here the expression of a bacterial sphingomyelinase in mammalian cells as a functionally active form. A chimeric Pseudomonas sphingomyelinase fused with the lysosomal sorting motif of lysosomal acid phosphatase was sorted to lysosomes in mammalian cells. As expected, the chimeric SMase hydrolyzed sphingomyelin in vivo to produce ceramide, part of which was converted to glucosylceramide.

元の言語英語
ページ(範囲)603-606
ページ数4
ジャーナルBioscience, Biotechnology and Biochemistry
71
発行部数2
DOI
出版物ステータス出版済み - 3 1 2007

    フィンガープリント

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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