Expression of active and inactive recombinant soluble trehalase using baculovirus-silkworm expression system and their glycan structures

Minoru Ujita, Mayumi Yamanaka, Yumiko Maeno, Koki Yoshida, Wakana Ohshio, Yoshinori Ueno, Yutaka Banno, Hiroshi Fujii, Hiroki Okumura

研究成果: ジャーナルへの寄稿記事

5 引用 (Scopus)

抄録

Silkworm soluble trehalase was expressed as a fusion protein with an N-terminal or C-terminal hexahistidine tag in a baculovirus-silkworm expression system and assayed for enzymatic activity. Only N-terminally tagged trehalase showed a high activity. This study is the first to report in vitro functional expression of recombinant insect soluble trehalase.

元の言語英語
ページ(範囲)22-25
ページ数4
ジャーナルJournal of Bioscience and Bioengineering
111
発行部数1
DOI
出版物ステータス出版済み - 1 1 2011

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Trehalase
Bombyx
Baculoviridae
Polysaccharides
His-His-His-His-His-His
Fusion reactions
Proteins
Insects

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

Expression of active and inactive recombinant soluble trehalase using baculovirus-silkworm expression system and their glycan structures. / Ujita, Minoru; Yamanaka, Mayumi; Maeno, Yumiko; Yoshida, Koki; Ohshio, Wakana; Ueno, Yoshinori; Banno, Yutaka; Fujii, Hiroshi; Okumura, Hiroki.

:: Journal of Bioscience and Bioengineering, 巻 111, 番号 1, 01.01.2011, p. 22-25.

研究成果: ジャーナルへの寄稿記事

Ujita, Minoru ; Yamanaka, Mayumi ; Maeno, Yumiko ; Yoshida, Koki ; Ohshio, Wakana ; Ueno, Yoshinori ; Banno, Yutaka ; Fujii, Hiroshi ; Okumura, Hiroki. / Expression of active and inactive recombinant soluble trehalase using baculovirus-silkworm expression system and their glycan structures. :: Journal of Bioscience and Bioengineering. 2011 ; 巻 111, 番号 1. pp. 22-25.
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AU - Ohshio, Wakana

AU - Ueno, Yoshinori

AU - Banno, Yutaka

AU - Fujii, Hiroshi

AU - Okumura, Hiroki

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