Expression of Antigen Genes of Leptospira interrogans Serovar canicola in Escherichia coli

Tsuyoshi Yamaguchi, Etsuro Ono, Ryo Yanagawa

研究成果: Contribution to journalArticle査読

7 被引用数 (Scopus)

抄録

Leptospira interrogans serovar canicola DNA was cloned into the plasmid pBR322 and introduced into E. coli. Eight out of approximately 10,000 transformants were found to express antigens of canicola by ELISA including colony ELISA blot test using anti-canicola antiserum. The canicola antigens expressed in the transformants reacted with the antisera against the serovars belonging to Canicola serogroup and other serogroups of L. interrogans. They did not react, however, with the antiserum against L. biflexa (with only one exception) nor with the antiserum against Leptonema illini. Thus, the recombinant DNA technique may provide alternative possibilities for preparing antigens of leptospires.

本文言語英語
ページ(範囲)1179-1187
ページ数9
ジャーナルMICROBIOLOGY and IMMUNOLOGY
32
12
DOI
出版ステータス出版済み - 1988
外部発表はい

All Science Journal Classification (ASJC) codes

  • 微生物学
  • 免疫学
  • ウイルス学

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