Expression of DCC and netrin-1 in normal human endometrium and its implication in endometrial carcinogenesis

H. D. Kato, H. Kondoh, T. Inoue, K. Asanoma, T. Matsuda, T. Arima, K. Kato, T. Yoshikawa, N. Wake

研究成果: Contribution to journalArticle査読

6 被引用数 (Scopus)

抄録

Although DCC has been considered as a candidate tumor suppressor, the roles it plays in the uterine endometrium and in the carcinogenic process remains unclear. To define these roles more clearly, we examined the expression of DCC and its ligand, netrin-1, in the normal endometrium and in endometrial cancer. The expression of DCC and netrin-1 in normal endometrial glands and in cancer cell lines was examined by RT-PCR and immunohistochemistry. The effects of exogenous DCC and netrin-1 expression were observed together with the respective expression vector transfection. Endometrial glands in the proliferative and early secretory phase expressed both DCC and netrin-1, but glands in the late-secretory phase tended to silence DCC expression. In addition, all of the endometrial cancer cell lines lost normal DCC expression. Restored DCC expression in the cancer cell lines in the absence of netrin-1 induced apoptosis. However, no changes were observed in the presence of netrin-1. Our observations suggest that DCC/netrin-1 signaling may commit cells to the transition of endometrial gland architecture or function from a proliferating to a secretory phase. In addition, the silencing of DCC expression may contribute to the escape of endometrial cancer cells from a DCC-regulated apoptotic program, thereby promoting malignant phenotypes.

本文言語英語
ページ(範囲)281-289
ページ数9
ジャーナルGynecologic Oncology
95
2
DOI
出版ステータス出版済み - 11 2004
外部発表はい

All Science Journal Classification (ASJC) codes

  • Oncology
  • Obstetrics and Gynaecology

フィンガープリント 「Expression of DCC and netrin-1 in normal human endometrium and its implication in endometrial carcinogenesis」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル