Expression of Hqk encoding a KH RNA binding protein is altered in human glioma

Hideki Nagashima, Noriaki Sadanaga, Kohjiro Mashino, Keishi Yamashita, Hiroshi Inoue, Masaki Mori, Keizo Sugimachi

研究成果: Contribution to journalArticle査読

48 被引用数 (Scopus)

抄録

The quaking gene family encodes single KH domain RNA-binding proteins that play vital roles in cell differentiation, proliferation, and apoptotic processes. The human quaking gene, Hqk, maps to 6q25-q26, where cytogenetic alterations associated with a variety of human malignancies, including gliomas have been reported. To assess possible relationships of Hqk with human diseases such as glial tumors, we first isolated the Hqk gene, characterized its structure and expression pattern, and carried out mutational analysis of Hqk in primary tumor samples. The Hqk gene contains 8 exons spanning a ∼200 kb genomic region, and generating at least four alternatively spliced transcripts, Hqk-5, Hqk-6, Hqk-7 and Hqk-7B, of which Hqk-7 is abundantly expressed in brain. Analysis of primary tumors demonstrated a high incidence of expression alterations of Hqk in gliomas (30%; 6/20), but not in other tumors such as schwannomas (0/3), or meningiomas (0/8). Among the tumor samples showing expression alterations, two were devoid of all three major transcripts, one was missing only the Hqk-5 message, and only the Hqk-7 message was absent in two cases. Our results thus imply the involvement of Hqk in human glial tumor progression.

本文言語英語
ページ(範囲)167-177
ページ数11
ジャーナルJapanese Journal of Cancer Research
93
2
DOI
出版ステータス出版済み - 2002

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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