Expression of human IL-13 receptor α2 extracellular domain in Pichia pastoris

Takatoshi Ohkuri, Chika Takeda, Yuichiro Yoshida, Kenji Izuhara, Taiji Imoto, Tadashi Ueda

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

Interleukin-13 receptor α2 (IL-13Rα2) binds IL-13 with high affinity and plays an important role in IL-13 signaling as a decoy receptor. We expressed the extracellular domain of human IL-13Rα2 (1-313) in methylotrophic yeast Pichia pastoris. SDS-PAGE analysis by PAS staining and Western blot analysis detected the product of the extracellular domain of human IL-13Rα2 as glycoprotein from P. pastoris. The yield of purified extracellular domain of human IL-13Rα2 was 2 mg from 1 L of culture. From CD analysis, the 2D structure of the purified IL-13Rα2 showed the typical β-sheet. ELISA of the purified IL-13Rα2 detected the binding activity for human IL-13. Thus, it was found that the active extracellular domain of human IL-13Rα2 was expressed from P. pastoris.

元の言語英語
ページ(範囲)48-53
ページ数6
ジャーナルProtein Expression and Purification
56
発行部数1
DOI
出版物ステータス出版済み - 11 1 2007

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Interleukin-13 Receptors
Pichia
Interleukin-13
P-Glycoprotein
Yeast
Polyacrylamide Gel Electrophoresis
Yeasts
Western Blotting
Enzyme-Linked Immunosorbent Assay
Staining and Labeling
human interleukin-13 receptor
human interleukin-13

All Science Journal Classification (ASJC) codes

  • Biotechnology

これを引用

Expression of human IL-13 receptor α2 extracellular domain in Pichia pastoris. / Ohkuri, Takatoshi; Takeda, Chika; Yoshida, Yuichiro; Izuhara, Kenji; Imoto, Taiji; Ueda, Tadashi.

:: Protein Expression and Purification, 巻 56, 番号 1, 01.11.2007, p. 48-53.

研究成果: ジャーナルへの寄稿記事

Ohkuri, Takatoshi ; Takeda, Chika ; Yoshida, Yuichiro ; Izuhara, Kenji ; Imoto, Taiji ; Ueda, Tadashi. / Expression of human IL-13 receptor α2 extracellular domain in Pichia pastoris. :: Protein Expression and Purification. 2007 ; 巻 56, 番号 1. pp. 48-53.
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AU - Ueda, Tadashi

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