TY - JOUR
T1 - Expression of MMP-8 and MMP-13 genes in the periodontal ligament during tooth movement in rats
AU - Takahashi, I.
AU - Nishimura, M.
AU - Onodera, K.
AU - Bae, J. W.
AU - Mitani, H.
AU - Okazaki, M.
AU - Sasano, Y.
AU - Mitani, H.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2003/8
Y1 - 2003/8
N2 - Periodontal ligament tissue is remodeled on both the tension and compression sides of moving teeth during orthodontic tooth movement. The present study was designed to clarify the hypothesis that the expression of MMP-8 and MMP-13 mRNA is promoted during the remodeling of periodontal ligament tissue in orthodontic tooth movement. We used the in situ hybridization method and semi-quantitative reverse-transcription/polymerase chain-reaction analysis to elucidate the gene expression of MMP-8 and MMP-13 mRNA. Expression of MMP-8 and MMP-13 mRNA transiently increased on both the compression and tension sides during active tooth movement in vivo. The gene expression of MMP-8 and MMP-13 was induced by tension, while compression indirectly promoted the gene expression of MMP-8 and MMP-13 through soluble factors in vitro. Thus, we concluded that the expression of MMP-8 and MMP-13 is differentially regulated by tension and compression, and plays an important role in the remodeling of the periodontal ligament.
AB - Periodontal ligament tissue is remodeled on both the tension and compression sides of moving teeth during orthodontic tooth movement. The present study was designed to clarify the hypothesis that the expression of MMP-8 and MMP-13 mRNA is promoted during the remodeling of periodontal ligament tissue in orthodontic tooth movement. We used the in situ hybridization method and semi-quantitative reverse-transcription/polymerase chain-reaction analysis to elucidate the gene expression of MMP-8 and MMP-13 mRNA. Expression of MMP-8 and MMP-13 mRNA transiently increased on both the compression and tension sides during active tooth movement in vivo. The gene expression of MMP-8 and MMP-13 was induced by tension, while compression indirectly promoted the gene expression of MMP-8 and MMP-13 through soluble factors in vitro. Thus, we concluded that the expression of MMP-8 and MMP-13 is differentially regulated by tension and compression, and plays an important role in the remodeling of the periodontal ligament.
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U2 - 10.1177/154405910308200815
DO - 10.1177/154405910308200815
M3 - Article
C2 - 12885852
AN - SCOPUS:0042887511
SN - 0022-0345
VL - 82
SP - 646
EP - 651
JO - Journal of Dental Research
JF - Journal of Dental Research
IS - 8
ER -