抄録
Background: High-risk human papillomavirus (hrHPV) type E6 and E7 oncoproteins contribute to oncogenesis in multiple ways by modulating the activities of host components in cell-cycle regulation including the expression of p16 protein (p16) and human telomerase reverse transcriptase (hTERT). The expression of p16 and hTERT protein in Bowenoid papulosis (BP) has not been studied. Methods: Biopsy samples of BP from 26 patients were subjected to in situ hybridization for various HPV strains and immunohistochemical staining for p16 and hTERT. Results: Among the 26 biopsy specimens, in situ hybridization using DNA probes for HPV 16/18 revealed positivity in 18 specimens (69.2%), one of which also showed positivity with the probes for HPV 6/11. HPV 31/33/35 was found in three specimens (11.5%). Two specimens (7.7%) were positive for unclassified HPV. Twenty-one BP specimens that were infected with hrHPV were positive for p16 and/or hTERT. Moderate or strong and diffuse immunostaining was observed for p16 in 15 hrHPV-infected specimens and for hTERT in 16 hrHPV-infected specimens. The expression of p16 or hTERT was each significantly associated with the presence of hrHPV. Conclusions: hrHPVs were involved in inducing p16 and hTERT overexpression in BP. Moreover, our results suggested that immunohistochemical p16 and hTERT expression might be a useful marker of hrHPV infection in BP.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 551-558 |
| ページ数 | 8 |
| ジャーナル | Journal of Cutaneous Pathology |
| 巻 | 33 |
| 発行部数 | 8 |
| DOI | |
| 出版物ステータス | 出版済み - 8 1 2006 |
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All Science Journal Classification (ASJC) codes
- Pathology and Forensic Medicine
- Histology
- Dermatology
これを引用
Expression of p16 and hTERT protein is associated with the presence of high-risk human papillomavirus in Bowenoid papulosis. / Liu, Houjun; Urabe, Kazunori; Moroi, Yoichi; Yasumoto, Shinichiro; Kokuba, Hisashi; Imafuku, Shinichi; Koga, Tetsuya; Masuda, Teiichi; Aburatani, Hiroyuki; Furue, Masutaka; Tu, Yating.
：: Journal of Cutaneous Pathology, 巻 33, 番号 8, 01.08.2006, p. 551-558.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Expression of p16 and hTERT protein is associated with the presence of high-risk human papillomavirus in Bowenoid papulosis
AU - Liu, Houjun
AU - Urabe, Kazunori
AU - Moroi, Yoichi
AU - Yasumoto, Shinichiro
AU - Kokuba, Hisashi
AU - Imafuku, Shinichi
AU - Koga, Tetsuya
AU - Masuda, Teiichi
AU - Aburatani, Hiroyuki
AU - Furue, Masutaka
AU - Tu, Yating
PY - 2006/8/1
Y1 - 2006/8/1
N2 - Background: High-risk human papillomavirus (hrHPV) type E6 and E7 oncoproteins contribute to oncogenesis in multiple ways by modulating the activities of host components in cell-cycle regulation including the expression of p16 protein (p16) and human telomerase reverse transcriptase (hTERT). The expression of p16 and hTERT protein in Bowenoid papulosis (BP) has not been studied. Methods: Biopsy samples of BP from 26 patients were subjected to in situ hybridization for various HPV strains and immunohistochemical staining for p16 and hTERT. Results: Among the 26 biopsy specimens, in situ hybridization using DNA probes for HPV 16/18 revealed positivity in 18 specimens (69.2%), one of which also showed positivity with the probes for HPV 6/11. HPV 31/33/35 was found in three specimens (11.5%). Two specimens (7.7%) were positive for unclassified HPV. Twenty-one BP specimens that were infected with hrHPV were positive for p16 and/or hTERT. Moderate or strong and diffuse immunostaining was observed for p16 in 15 hrHPV-infected specimens and for hTERT in 16 hrHPV-infected specimens. The expression of p16 or hTERT was each significantly associated with the presence of hrHPV. Conclusions: hrHPVs were involved in inducing p16 and hTERT overexpression in BP. Moreover, our results suggested that immunohistochemical p16 and hTERT expression might be a useful marker of hrHPV infection in BP.
AB - Background: High-risk human papillomavirus (hrHPV) type E6 and E7 oncoproteins contribute to oncogenesis in multiple ways by modulating the activities of host components in cell-cycle regulation including the expression of p16 protein (p16) and human telomerase reverse transcriptase (hTERT). The expression of p16 and hTERT protein in Bowenoid papulosis (BP) has not been studied. Methods: Biopsy samples of BP from 26 patients were subjected to in situ hybridization for various HPV strains and immunohistochemical staining for p16 and hTERT. Results: Among the 26 biopsy specimens, in situ hybridization using DNA probes for HPV 16/18 revealed positivity in 18 specimens (69.2%), one of which also showed positivity with the probes for HPV 6/11. HPV 31/33/35 was found in three specimens (11.5%). Two specimens (7.7%) were positive for unclassified HPV. Twenty-one BP specimens that were infected with hrHPV were positive for p16 and/or hTERT. Moderate or strong and diffuse immunostaining was observed for p16 in 15 hrHPV-infected specimens and for hTERT in 16 hrHPV-infected specimens. The expression of p16 or hTERT was each significantly associated with the presence of hrHPV. Conclusions: hrHPVs were involved in inducing p16 and hTERT overexpression in BP. Moreover, our results suggested that immunohistochemical p16 and hTERT expression might be a useful marker of hrHPV infection in BP.
UR - http://www.scopus.com/inward/record.url?scp=33747042751&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33747042751&partnerID=8YFLogxK
U2 - 10.1111/j.1600-0560.2006.00438.x
DO - 10.1111/j.1600-0560.2006.00438.x
M3 - Article
C2 - 16919029
AN - SCOPUS:33747042751
VL - 33
SP - 551
EP - 558
JO - Journal of Cutaneous Pathology
JF - Journal of Cutaneous Pathology
SN - 0303-6987
IS - 8
ER -