Expression of peroxisome proliferator-activated receptor isoforms in the rat uterus during early pregnancy

Kyohei Nishimura, Nobuhiko Yamauchi, Vishwajit Surchowdhury, Mikinori Torii, Masa Aki Hattori, Masako Kaneto

研究成果: ジャーナルへの寄稿記事

11 引用 (Scopus)

抄録

Peroxisome proliferator-activated receptors (PPARs) play an important role in different compartments of the female reproductive system in rodents and humans. However, expressional profiles and physiological functions of PPARs in the endometrium prior to the placentation are not well understood. In this study, we determined expressional profiles of the PPARs during early pregnancy. Immunocytochemistry revealed that both PPARα and PPARβ/δ were strongly detected in the endometrial stroma on days 4.5-6.5 of pregnancy, which is just a starting time of implantation. Delayed implantation animal model showed that the expressions of PPARα and PPARβ/δ occurred after the initiation of implantation in the endometrial stroma. Moreover, an in vitro decidualization model further revealed that the expression of PPARα increased in the cultured rat endometrial stromal cells at 24 h after the decidualization treatment, but the expression of PPARβ/δ was delayed and increased at 48 h after the treatment. PPARγ was expressed in the endometrial stroma and its expression decreased significantly at 2.5 days post-coitum and maintained a low level of expression during the period of implantation. These results indicate that PPARα is expressed and induced by the initiation of implantation, prior to the expression of PPARβ/δ in decidualized endometrium. Increasing expression of PPARγ during fertilization and its decline during the period of implantation further suggest that PPARs may play important roles during early pregnancy.

元の言語英語
ページ(範囲)275-284
ページ数10
ジャーナルCell and tissue research
345
発行部数2
DOI
出版物ステータス出版済み - 8 1 2011

Fingerprint

Peroxisome Proliferator-Activated Receptors
Uterus
Protein Isoforms
Pregnancy
Endometrium
Placentation
Stromal Cells
Fertilization

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

これを引用

Expression of peroxisome proliferator-activated receptor isoforms in the rat uterus during early pregnancy. / Nishimura, Kyohei; Yamauchi, Nobuhiko; Surchowdhury, Vishwajit; Torii, Mikinori; Hattori, Masa Aki; Kaneto, Masako.

:: Cell and tissue research, 巻 345, 番号 2, 01.08.2011, p. 275-284.

研究成果: ジャーナルへの寄稿記事

Nishimura, Kyohei ; Yamauchi, Nobuhiko ; Surchowdhury, Vishwajit ; Torii, Mikinori ; Hattori, Masa Aki ; Kaneto, Masako. / Expression of peroxisome proliferator-activated receptor isoforms in the rat uterus during early pregnancy. :: Cell and tissue research. 2011 ; 巻 345, 番号 2. pp. 275-284.
@article{0ad98dfe889a4f369fd365162f704bf5,
title = "Expression of peroxisome proliferator-activated receptor isoforms in the rat uterus during early pregnancy",
abstract = "Peroxisome proliferator-activated receptors (PPARs) play an important role in different compartments of the female reproductive system in rodents and humans. However, expressional profiles and physiological functions of PPARs in the endometrium prior to the placentation are not well understood. In this study, we determined expressional profiles of the PPARs during early pregnancy. Immunocytochemistry revealed that both PPARα and PPARβ/δ were strongly detected in the endometrial stroma on days 4.5-6.5 of pregnancy, which is just a starting time of implantation. Delayed implantation animal model showed that the expressions of PPARα and PPARβ/δ occurred after the initiation of implantation in the endometrial stroma. Moreover, an in vitro decidualization model further revealed that the expression of PPARα increased in the cultured rat endometrial stromal cells at 24 h after the decidualization treatment, but the expression of PPARβ/δ was delayed and increased at 48 h after the treatment. PPARγ was expressed in the endometrial stroma and its expression decreased significantly at 2.5 days post-coitum and maintained a low level of expression during the period of implantation. These results indicate that PPARα is expressed and induced by the initiation of implantation, prior to the expression of PPARβ/δ in decidualized endometrium. Increasing expression of PPARγ during fertilization and its decline during the period of implantation further suggest that PPARs may play important roles during early pregnancy.",
author = "Kyohei Nishimura and Nobuhiko Yamauchi and Vishwajit Surchowdhury and Mikinori Torii and Hattori, {Masa Aki} and Masako Kaneto",
year = "2011",
month = "8",
day = "1",
doi = "10.1007/s00441-011-1208-4",
language = "English",
volume = "345",
pages = "275--284",
journal = "Cell and Tissue Research",
issn = "0302-766X",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - Expression of peroxisome proliferator-activated receptor isoforms in the rat uterus during early pregnancy

AU - Nishimura, Kyohei

AU - Yamauchi, Nobuhiko

AU - Surchowdhury, Vishwajit

AU - Torii, Mikinori

AU - Hattori, Masa Aki

AU - Kaneto, Masako

PY - 2011/8/1

Y1 - 2011/8/1

N2 - Peroxisome proliferator-activated receptors (PPARs) play an important role in different compartments of the female reproductive system in rodents and humans. However, expressional profiles and physiological functions of PPARs in the endometrium prior to the placentation are not well understood. In this study, we determined expressional profiles of the PPARs during early pregnancy. Immunocytochemistry revealed that both PPARα and PPARβ/δ were strongly detected in the endometrial stroma on days 4.5-6.5 of pregnancy, which is just a starting time of implantation. Delayed implantation animal model showed that the expressions of PPARα and PPARβ/δ occurred after the initiation of implantation in the endometrial stroma. Moreover, an in vitro decidualization model further revealed that the expression of PPARα increased in the cultured rat endometrial stromal cells at 24 h after the decidualization treatment, but the expression of PPARβ/δ was delayed and increased at 48 h after the treatment. PPARγ was expressed in the endometrial stroma and its expression decreased significantly at 2.5 days post-coitum and maintained a low level of expression during the period of implantation. These results indicate that PPARα is expressed and induced by the initiation of implantation, prior to the expression of PPARβ/δ in decidualized endometrium. Increasing expression of PPARγ during fertilization and its decline during the period of implantation further suggest that PPARs may play important roles during early pregnancy.

AB - Peroxisome proliferator-activated receptors (PPARs) play an important role in different compartments of the female reproductive system in rodents and humans. However, expressional profiles and physiological functions of PPARs in the endometrium prior to the placentation are not well understood. In this study, we determined expressional profiles of the PPARs during early pregnancy. Immunocytochemistry revealed that both PPARα and PPARβ/δ were strongly detected in the endometrial stroma on days 4.5-6.5 of pregnancy, which is just a starting time of implantation. Delayed implantation animal model showed that the expressions of PPARα and PPARβ/δ occurred after the initiation of implantation in the endometrial stroma. Moreover, an in vitro decidualization model further revealed that the expression of PPARα increased in the cultured rat endometrial stromal cells at 24 h after the decidualization treatment, but the expression of PPARβ/δ was delayed and increased at 48 h after the treatment. PPARγ was expressed in the endometrial stroma and its expression decreased significantly at 2.5 days post-coitum and maintained a low level of expression during the period of implantation. These results indicate that PPARα is expressed and induced by the initiation of implantation, prior to the expression of PPARβ/δ in decidualized endometrium. Increasing expression of PPARγ during fertilization and its decline during the period of implantation further suggest that PPARs may play important roles during early pregnancy.

UR - http://www.scopus.com/inward/record.url?scp=80052772363&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052772363&partnerID=8YFLogxK

U2 - 10.1007/s00441-011-1208-4

DO - 10.1007/s00441-011-1208-4

M3 - Article

C2 - 21773887

AN - SCOPUS:80052772363

VL - 345

SP - 275

EP - 284

JO - Cell and Tissue Research

JF - Cell and Tissue Research

SN - 0302-766X

IS - 2

ER -