Expression of prophenoloxidase mRNA during silkworm hemocyte development

Kohji Yamamoto, Masatoshi Yakiyama, Hiroshi Fujii, Takahiro Kusakabe, Katsumi Koga, Yoichi Aso, Masatsune Ishiguro

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

Two clones encoding different prophenoloxidase isoforms were amplified by polymerase chain reaction of RNA from the hemocytes of an experimental strain of Bombyx mori. The nucleotide sequences of the clones and the deduced amino acid sequences were confirmed to be nearly identical to those of the orthologous clones previously obtained from a commercial race of B. mori. Northern blot hybridization using these clones as probes demonstrated that the prophenoloxidase mRNA in the hemocytes is expressed in a stage-specific manner during the final larval instar and pupal stage, showing a peak one day before pupation in males and on the day of pupation in females. A sexual difference was also observed when the content of prophenoloxidase protein in the hemolymph (including hemocytes) was measured by an enzyme-linked immunosorbent assay.

元の言語英語
ページ(範囲)1197-1202
ページ数6
ジャーナルBioscience, Biotechnology and Biochemistry
64
発行部数6
DOI
出版物ステータス出版済み - 1 1 2000

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Hemocytes
Bombyx
Clone Cells
Messenger RNA
Immunosorbents
Polymerase chain reaction
Hemolymph
Assays
Protein Isoforms
Nucleotides
Northern Blotting
RNA
Amino Acids
Amino Acid Sequence
Enzyme-Linked Immunosorbent Assay
Enzymes
Polymerase Chain Reaction
pro-phenoloxidase
Proteins

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

これを引用

Expression of prophenoloxidase mRNA during silkworm hemocyte development. / Yamamoto, Kohji; Yakiyama, Masatoshi; Fujii, Hiroshi; Kusakabe, Takahiro; Koga, Katsumi; Aso, Yoichi; Ishiguro, Masatsune.

:: Bioscience, Biotechnology and Biochemistry, 巻 64, 番号 6, 01.01.2000, p. 1197-1202.

研究成果: ジャーナルへの寄稿記事

Yamamoto, Kohji ; Yakiyama, Masatoshi ; Fujii, Hiroshi ; Kusakabe, Takahiro ; Koga, Katsumi ; Aso, Yoichi ; Ishiguro, Masatsune. / Expression of prophenoloxidase mRNA during silkworm hemocyte development. :: Bioscience, Biotechnology and Biochemistry. 2000 ; 巻 64, 番号 6. pp. 1197-1202.
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