TY - JOUR
T1 - Expression of prothymosin-α and c-myc mRNA in human gastric cancer
AU - Shibuta, Kenji
AU - Mori, Masaki
AU - Mimori, Koshi
AU - Inoue, Hiroshi
AU - Nakashima, Hideaki
AU - Baba, Kinya
AU - Haraguchi, Masaru
AU - Karimine, Nobuya
AU - Ueo, Hiroaki
AU - Akiyoshi, Tsuyoshi
PY - 1996/8/1
Y1 - 1996/8/1
N2 - Prothymosin-α (PT-α) is a nuclear protein involved in cell proliferation. c-myc is implicated in the carcinogenesis of many human cancers. PT-α gene transcription is reported to be regulated by the c-myc gene in vitro. However, little has been reported on the PT-α and c-myc mRNA expressions in gastric cancer. We semi-quantitatively determined the PT-α and c-myc mRNA expressions in 60 pairs of gastric cancer tissue (T) and corresponding normal tissue (N) using the reverse transcriptase-polymerase chain reaction method. The average of T/N ratio was 1.20 for PT-α and 1.30 for c-myc. Cases demonstrating a T/N ratio of more than 1.0 were seen in 33 (55%) and 30 (50%) cases for PT-α and c-myc, respectively. No significant correlation was observed between either of these two mRNA expressions and any of the examined clinicopathologic factors for gastric cancer. However, a significant correlation was seen between the expressions of both genes (p < 0.0001). The findings support the hypothesis that, regarding human gastric cancer, the transcription of PT-α is considered to be under the control of c-myc gene, however, the value of these gene expressions do not reflect biological behavior.
AB - Prothymosin-α (PT-α) is a nuclear protein involved in cell proliferation. c-myc is implicated in the carcinogenesis of many human cancers. PT-α gene transcription is reported to be regulated by the c-myc gene in vitro. However, little has been reported on the PT-α and c-myc mRNA expressions in gastric cancer. We semi-quantitatively determined the PT-α and c-myc mRNA expressions in 60 pairs of gastric cancer tissue (T) and corresponding normal tissue (N) using the reverse transcriptase-polymerase chain reaction method. The average of T/N ratio was 1.20 for PT-α and 1.30 for c-myc. Cases demonstrating a T/N ratio of more than 1.0 were seen in 33 (55%) and 30 (50%) cases for PT-α and c-myc, respectively. No significant correlation was observed between either of these two mRNA expressions and any of the examined clinicopathologic factors for gastric cancer. However, a significant correlation was seen between the expressions of both genes (p < 0.0001). The findings support the hypothesis that, regarding human gastric cancer, the transcription of PT-α is considered to be under the control of c-myc gene, however, the value of these gene expressions do not reflect biological behavior.
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M3 - Article
C2 - 21541507
AN - SCOPUS:0030018845
VL - 9
SP - 247
EP - 251
JO - International Journal of Oncology
JF - International Journal of Oncology
SN - 1019-6439
IS - 2
ER -