Expression of prothymosin-α and c-myc mRNA in human gastric cancer

Prothymosin-α (PT-α) is a nuclear protein involved in cell proliferation. c-myc is implicated in the carcinogenesis of many human cancers. PT-α gene transcription is reported to be regulated by the c-myc gene in vitro. However, little has been reported on the PT-α and c-myc mRNA expressions in gastric cancer. We semi-quantitatively determined the PT-α and c-myc mRNA expressions in 60 pairs of gastric cancer tissue (T) and corresponding normal tissue (N) using the reverse transcriptase-polymerase chain reaction method. The average of T/N ratio was 1.20 for PT-α and 1.30 for c-myc. Cases demonstrating a T/N ratio of more than 1.0 were seen in 33 (55%) and 30 (50%) cases for PT-α and c-myc, respectively. No significant correlation was observed between either of these two mRNA expressions and any of the examined clinicopathologic factors for gastric cancer. However, a significant correlation was seen between the expressions of both genes (p < 0.0001). The findings support the hypothesis that, regarding human gastric cancer, the transcription of PT-α is considered to be under the control of c-myc gene, however, the value of these gene expressions do not reflect biological behavior.