Expression of the c-myc and c-fos genes as a potential indicator of late radiation damage to the kidney

Makoto Otsuka, Masamitsu Hatakenaka, Kousei Ishigami, Kouji Masuda

研究成果: Contribution to journalArticle査読

5 被引用数 (Scopus)

抄録

Purpose: To evaluate proliferative response according to c-myc and c-fos gene expression as an early indicator of late radiation damage to the kidney. Methods and Materials: c-myc and c-fos gene expression was measured in the irradiated kidney of C3H/HeSlc mice using reverse transcriptase-polymerase chain reaction (RT-PCR) 24 h after 9, 12, and 15 Gy as well as 2 and 7 days after 15 Gy. In a second experiment, 12 Gy was administered to the right kidney plus the lower half of left kidney. The mice underwent left nephrectomy 24 h after, and the correlation between increased gene expression and elevation of the blood urea nitrogen (BUN) level representing late functional damage was investigated. Results: c-myc and c-fos expression increased in 1 of 10 controls and in 8 of 10 and 7 of 10 mice that received 15 Gy, respectively. Expression increased dose-dependently from 9 to 15 Gy. In mice having an increased BUN level, 7 of 10 and 8 of 10 were positive for c-myc and c-fos, respectively. All 6 mice having an unchanged BUN level were negative for c-fos, while 5 were negative for c-myc. Conclusion: Measurement of c-myc and c-fos gene expression is potentially an early indicator of late radiation damage to the kidney.

本文言語英語
ページ(範囲)169-173
ページ数5
ジャーナルInternational Journal of Radiation Oncology Biology Physics
49
1
DOI
出版ステータス出版済み - 1 1 2001

All Science Journal Classification (ASJC) codes

  • 放射線
  • 腫瘍学
  • 放射線学、核医学およびイメージング
  • 癌研究

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