Expression of the human MHC, HLA-DQw6 genes alters the immune response in C57BL/6 mice

Y. Nishimura, T. Iwanaga, T. Inamitsu, Y. Yanagawa, M. Yasunami, A. Kimura, K. Hirokawa, Takehiko Sasazuki

研究成果: ジャーナルへの寄稿記事

31 引用 (Scopus)

抄録

In an attempt to obtain direct evidence for the critical role of HLA class II molecules in regulating the immune response, genomic genes for α- and β-chains of HLA-DQw6 from HLA-Dw12 haplotype were introduced into the C57BL/6 (B6) strain of mouse and a line of HLA-DQw6 transgenic mouse was obtained. Tissue specificity of the expression of the transgenes was much the same as that of murine L-Ab genes. DQw6 molecules were expressed on B cells and macrophages in spleen cells and about 30 to 40% of the I-Ab+ spleen cells were positive for DQw6. The HLA-DQw6 transgenic B6 mouse became tolerant to the DQw6 molecules, as evidenced by the MLR and antibody production specific to the DQw6 molecules. The HLA-DQw6 transgenic B6 mouse showed a strong immune response to streptococcal cell wall antigen (SCW), whereas the B6 mouse was a low responder to SCW. The SCW-specific T cell line was established from the transgenic mouse and this T cell line recognized SCW in the context of HLA-DQw6 molecules expressed on the mouse L cell transfectant or on human monocytes. The proliferative response to SCW of primed lymph node T cells and the SCW-specific T cell line derived from the transgenic mice was inhibited by anti-HLA-DQ mAb. Thus, it is clear that the HLA-DQw6 genes acted as major histocompatibility genes in these transgenic mice.

元の言語英語
ページ(範囲)353-360
ページ数8
ジャーナルJournal of Immunology
145
発行部数1
出版物ステータス出版済み - 1990

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Inbred C57BL Mouse
Cell Wall
Transgenic Mice
Antigens
Genes
T-Lymphocytes
Cell Line
Spleen
HLA-DQ Antigens
Organ Specificity
Histocompatibility
Transgenes
Haplotypes
Antibody Formation
HLA-DQ6 antigen
Monocytes
B-Lymphocytes
Lymph Nodes
Macrophages

All Science Journal Classification (ASJC) codes

  • Immunology

これを引用

Nishimura, Y., Iwanaga, T., Inamitsu, T., Yanagawa, Y., Yasunami, M., Kimura, A., ... Sasazuki, T. (1990). Expression of the human MHC, HLA-DQw6 genes alters the immune response in C57BL/6 mice. Journal of Immunology, 145(1), 353-360.

Expression of the human MHC, HLA-DQw6 genes alters the immune response in C57BL/6 mice. / Nishimura, Y.; Iwanaga, T.; Inamitsu, T.; Yanagawa, Y.; Yasunami, M.; Kimura, A.; Hirokawa, K.; Sasazuki, Takehiko.

:: Journal of Immunology, 巻 145, 番号 1, 1990, p. 353-360.

研究成果: ジャーナルへの寄稿記事

Nishimura, Y, Iwanaga, T, Inamitsu, T, Yanagawa, Y, Yasunami, M, Kimura, A, Hirokawa, K & Sasazuki, T 1990, 'Expression of the human MHC, HLA-DQw6 genes alters the immune response in C57BL/6 mice', Journal of Immunology, 巻. 145, 番号 1, pp. 353-360.
Nishimura Y, Iwanaga T, Inamitsu T, Yanagawa Y, Yasunami M, Kimura A その他. Expression of the human MHC, HLA-DQw6 genes alters the immune response in C57BL/6 mice. Journal of Immunology. 1990;145(1):353-360.
Nishimura, Y. ; Iwanaga, T. ; Inamitsu, T. ; Yanagawa, Y. ; Yasunami, M. ; Kimura, A. ; Hirokawa, K. ; Sasazuki, Takehiko. / Expression of the human MHC, HLA-DQw6 genes alters the immune response in C57BL/6 mice. :: Journal of Immunology. 1990 ; 巻 145, 番号 1. pp. 353-360.
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abstract = "In an attempt to obtain direct evidence for the critical role of HLA class II molecules in regulating the immune response, genomic genes for α- and β-chains of HLA-DQw6 from HLA-Dw12 haplotype were introduced into the C57BL/6 (B6) strain of mouse and a line of HLA-DQw6 transgenic mouse was obtained. Tissue specificity of the expression of the transgenes was much the same as that of murine L-Ab genes. DQw6 molecules were expressed on B cells and macrophages in spleen cells and about 30 to 40{\%} of the I-Ab+ spleen cells were positive for DQw6. The HLA-DQw6 transgenic B6 mouse became tolerant to the DQw6 molecules, as evidenced by the MLR and antibody production specific to the DQw6 molecules. The HLA-DQw6 transgenic B6 mouse showed a strong immune response to streptococcal cell wall antigen (SCW), whereas the B6 mouse was a low responder to SCW. The SCW-specific T cell line was established from the transgenic mouse and this T cell line recognized SCW in the context of HLA-DQw6 molecules expressed on the mouse L cell transfectant or on human monocytes. The proliferative response to SCW of primed lymph node T cells and the SCW-specific T cell line derived from the transgenic mice was inhibited by anti-HLA-DQ mAb. Thus, it is clear that the HLA-DQw6 genes acted as major histocompatibility genes in these transgenic mice.",
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AU - Yasunami, M.

AU - Kimura, A.

AU - Hirokawa, K.

AU - Sasazuki, Takehiko

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