TY - JOUR
T1 - Expression of two kinds of cytochrome P-450(11β) mrna in bovine adrenal cortex
AU - Kirita, Shirou
AU - Morohashi, Ken ichirou
AU - Hashimoto, Toshihide
AU - Yoshioka, Hidefumi
AU - Fujii-kuriyama, Yoshiaki
AU - Omura, Tsuneo
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1988/11
Y1 - 1988/11
N2 - Using pcP-450(11β)-2 cDNA (Morohashi et al. (1987) J. Biochem. 102, 559-568) as the probe, a different cDNA clone, pcP-450(11β)-3, was isolated from a cDNA library of bovine adrenal cortex. The restriction enzyme map of pcP-450(11β)-3 was highly homologous but not identical with that of pcP-450(11β)-2. Nucleotide sequence determination revealed the substitutions of 14 nucleotides and 3 amino acids between pcP-450(11β)-2 and -3. Blotting analysis involving two different oligonucleotide probes specific to these two cDNAs indicated that at least two kinds of P-450(11β) mRNA were expressed in individual animals and that a least two kinds of P-450(11β) genes exist in the bovine genome.
AB - Using pcP-450(11β)-2 cDNA (Morohashi et al. (1987) J. Biochem. 102, 559-568) as the probe, a different cDNA clone, pcP-450(11β)-3, was isolated from a cDNA library of bovine adrenal cortex. The restriction enzyme map of pcP-450(11β)-3 was highly homologous but not identical with that of pcP-450(11β)-2. Nucleotide sequence determination revealed the substitutions of 14 nucleotides and 3 amino acids between pcP-450(11β)-2 and -3. Blotting analysis involving two different oligonucleotide probes specific to these two cDNAs indicated that at least two kinds of P-450(11β) mRNA were expressed in individual animals and that a least two kinds of P-450(11β) genes exist in the bovine genome.
UR - http://www.scopus.com/inward/record.url?scp=0023797396&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023797396&partnerID=8YFLogxK
U2 - 10.1093/oxfordjournals.jbchem.a122533
DO - 10.1093/oxfordjournals.jbchem.a122533
M3 - Article
C2 - 3266212
AN - SCOPUS:0023797396
SN - 0021-924X
VL - 104
SP - 683
EP - 686
JO - Journal of Biochemistry
JF - Journal of Biochemistry
IS - 5
ER -