Expression of two kinds of cytochrome P-450(11β) mrna in bovine adrenal cortex

Shirou Kirita, Ken ichirou Morohashi, Toshihide Hashimoto, Hidefumi Yoshioka, Yoshiaki Fujii-kuriyama, Tsuneo Omura

研究成果: Contribution to journalArticle査読

46 被引用数 (Scopus)

抄録

Using pcP-450(11β)-2 cDNA (Morohashi et al. (1987) J. Biochem. 102, 559-568) as the probe, a different cDNA clone, pcP-450(11β)-3, was isolated from a cDNA library of bovine adrenal cortex. The restriction enzyme map of pcP-450(11β)-3 was highly homologous but not identical with that of pcP-450(11β)-2. Nucleotide sequence determination revealed the substitutions of 14 nucleotides and 3 amino acids between pcP-450(11β)-2 and -3. Blotting analysis involving two different oligonucleotide probes specific to these two cDNAs indicated that at least two kinds of P-450(11β) mRNA were expressed in individual animals and that a least two kinds of P-450(11β) genes exist in the bovine genome.

本文言語英語
ページ(範囲)683-686
ページ数4
ジャーナルJournal of biochemistry
104
5
DOI
出版ステータス出版済み - 11 1988

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学

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