Expression profiles of COUP-TF, DAX-1, and SF-1 in the human adrenal gland and adrenocortical tumors: Possible implications in steroidogenesis

Hirotaka Shibata, Yayoi Ikeda, Tokuo Mukai, Ken-Ichirou Morohashi, Isao Kurihara, Takashi Ando, Toshihiko Suzuki, Sakiko Kobayashi, Masaru Murai, Ikuo Saito, Takao Saruta

研究成果: ジャーナルへの寄稿記事

59 引用 (Scopus)

抄録

Chicken ovalbumin upstream promoter-transcription factor (COUP-TF), DAX-1, and steroidogenic factor-1 (SF-1) are orphan members of the nuclear hormone receptor superfamily. COUP-TF and DAX-1 have been shown to negatively regulate the transcriptional activity of SF-1, a steroidogenic cell-specific activator of various steroidogenic cytochrome P450 genes. We therefore examined the expression levels and immunolocalization of COUP-TF, DAX-1, and SF-1 in human adrenal gland (NL) and adrenocortical adenomas, and compared the results with CYP17 expression levels and its enzyme activities to study their potential correlation with adrenocortical steroidogenesis. In NL (n=10), expressions of COUP-TF, DAX-1, and SF-1 were detected in the nuclei of adrenocortical cells, but not in the medulla. In cortisol-producing adenomas causing Cushing syndrome (CS, n=20), CYP17 expression was upregulated (298±2% vs NL 98 ± 4%), whereas expression levels of both COUP-TFs (COUP-TFI, 52±5% vs NL 98±4%; COUP-TFII, 18±4% vs NL 98±4%) and DAX-1 (42±4% vs NL 100±4%) were reduced. In deoxycorticosterone-producing adenomas (DOC, n=2), on the other hand, CYP17 expression was extremely reduced (8 and 12% vs NL 98±4%), whereas DAX-1 expression increased markedly (350 and 360% vs NL 100±4%). Expression levels of SF-1 did not differ between NL (100±8%) and CS (106±10%), but its expression appeared to be decreased in DOC (25 and 20%). These results showed CYP17 expression to be upregulated and downregulated in CS and DOC, respectively, in a manner reciprocal to that of its repressors, COUP-TF and/or DAX-1. In summary, the results indicate that co-localization of COUP-TF, DAX-1, and SF-1 in NL was lost in adrenocortical tumors and that these orphan receptors play an important role in the regulation of steroidogenesis in human adrenals.

元の言語英語
ページ(範囲)206-216
ページ数11
ジャーナルMolecular Genetics and Metabolism
74
発行部数1-2
DOI
出版物ステータス出版済み - 1 1 2001
外部発表Yes

Fingerprint

COUP Transcription Factors
Steroidogenic Factor 1
Adrenal Glands
Steroid 17-alpha-Hydroxylase
Tumors
Neoplasms
Adenoma
Adrenocortical Adenoma
Desoxycorticosterone
Cushing Syndrome
Enzyme activity
Cytoplasmic and Nuclear Receptors
Cell Nucleus
Cytochrome P-450 Enzyme System
Hydrocortisone
human NR5A1 protein
Down-Regulation
Genes
Enzymes

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Endocrinology

これを引用

Expression profiles of COUP-TF, DAX-1, and SF-1 in the human adrenal gland and adrenocortical tumors : Possible implications in steroidogenesis. / Shibata, Hirotaka; Ikeda, Yayoi; Mukai, Tokuo; Morohashi, Ken-Ichirou; Kurihara, Isao; Ando, Takashi; Suzuki, Toshihiko; Kobayashi, Sakiko; Murai, Masaru; Saito, Ikuo; Saruta, Takao.

:: Molecular Genetics and Metabolism, 巻 74, 番号 1-2, 01.01.2001, p. 206-216.

研究成果: ジャーナルへの寄稿記事

Shibata, H, Ikeda, Y, Mukai, T, Morohashi, K-I, Kurihara, I, Ando, T, Suzuki, T, Kobayashi, S, Murai, M, Saito, I & Saruta, T 2001, 'Expression profiles of COUP-TF, DAX-1, and SF-1 in the human adrenal gland and adrenocortical tumors: Possible implications in steroidogenesis', Molecular Genetics and Metabolism, 巻. 74, 番号 1-2, pp. 206-216. https://doi.org/10.1006/mgme.2001.3231
Shibata, Hirotaka ; Ikeda, Yayoi ; Mukai, Tokuo ; Morohashi, Ken-Ichirou ; Kurihara, Isao ; Ando, Takashi ; Suzuki, Toshihiko ; Kobayashi, Sakiko ; Murai, Masaru ; Saito, Ikuo ; Saruta, Takao. / Expression profiles of COUP-TF, DAX-1, and SF-1 in the human adrenal gland and adrenocortical tumors : Possible implications in steroidogenesis. :: Molecular Genetics and Metabolism. 2001 ; 巻 74, 番号 1-2. pp. 206-216.
@article{0f42f9f4a0a14f39b488d7715ef6c861,
title = "Expression profiles of COUP-TF, DAX-1, and SF-1 in the human adrenal gland and adrenocortical tumors: Possible implications in steroidogenesis",
abstract = "Chicken ovalbumin upstream promoter-transcription factor (COUP-TF), DAX-1, and steroidogenic factor-1 (SF-1) are orphan members of the nuclear hormone receptor superfamily. COUP-TF and DAX-1 have been shown to negatively regulate the transcriptional activity of SF-1, a steroidogenic cell-specific activator of various steroidogenic cytochrome P450 genes. We therefore examined the expression levels and immunolocalization of COUP-TF, DAX-1, and SF-1 in human adrenal gland (NL) and adrenocortical adenomas, and compared the results with CYP17 expression levels and its enzyme activities to study their potential correlation with adrenocortical steroidogenesis. In NL (n=10), expressions of COUP-TF, DAX-1, and SF-1 were detected in the nuclei of adrenocortical cells, but not in the medulla. In cortisol-producing adenomas causing Cushing syndrome (CS, n=20), CYP17 expression was upregulated (298±2{\%} vs NL 98 ± 4{\%}), whereas expression levels of both COUP-TFs (COUP-TFI, 52±5{\%} vs NL 98±4{\%}; COUP-TFII, 18±4{\%} vs NL 98±4{\%}) and DAX-1 (42±4{\%} vs NL 100±4{\%}) were reduced. In deoxycorticosterone-producing adenomas (DOC, n=2), on the other hand, CYP17 expression was extremely reduced (8 and 12{\%} vs NL 98±4{\%}), whereas DAX-1 expression increased markedly (350 and 360{\%} vs NL 100±4{\%}). Expression levels of SF-1 did not differ between NL (100±8{\%}) and CS (106±10{\%}), but its expression appeared to be decreased in DOC (25 and 20{\%}). These results showed CYP17 expression to be upregulated and downregulated in CS and DOC, respectively, in a manner reciprocal to that of its repressors, COUP-TF and/or DAX-1. In summary, the results indicate that co-localization of COUP-TF, DAX-1, and SF-1 in NL was lost in adrenocortical tumors and that these orphan receptors play an important role in the regulation of steroidogenesis in human adrenals.",
author = "Hirotaka Shibata and Yayoi Ikeda and Tokuo Mukai and Ken-Ichirou Morohashi and Isao Kurihara and Takashi Ando and Toshihiko Suzuki and Sakiko Kobayashi and Masaru Murai and Ikuo Saito and Takao Saruta",
year = "2001",
month = "1",
day = "1",
doi = "10.1006/mgme.2001.3231",
language = "English",
volume = "74",
pages = "206--216",
journal = "Molecular Genetics and Metabolism",
issn = "1096-7192",
publisher = "Academic Press Inc.",
number = "1-2",

}

TY - JOUR

T1 - Expression profiles of COUP-TF, DAX-1, and SF-1 in the human adrenal gland and adrenocortical tumors

T2 - Possible implications in steroidogenesis

AU - Shibata, Hirotaka

AU - Ikeda, Yayoi

AU - Mukai, Tokuo

AU - Morohashi, Ken-Ichirou

AU - Kurihara, Isao

AU - Ando, Takashi

AU - Suzuki, Toshihiko

AU - Kobayashi, Sakiko

AU - Murai, Masaru

AU - Saito, Ikuo

AU - Saruta, Takao

PY - 2001/1/1

Y1 - 2001/1/1

N2 - Chicken ovalbumin upstream promoter-transcription factor (COUP-TF), DAX-1, and steroidogenic factor-1 (SF-1) are orphan members of the nuclear hormone receptor superfamily. COUP-TF and DAX-1 have been shown to negatively regulate the transcriptional activity of SF-1, a steroidogenic cell-specific activator of various steroidogenic cytochrome P450 genes. We therefore examined the expression levels and immunolocalization of COUP-TF, DAX-1, and SF-1 in human adrenal gland (NL) and adrenocortical adenomas, and compared the results with CYP17 expression levels and its enzyme activities to study their potential correlation with adrenocortical steroidogenesis. In NL (n=10), expressions of COUP-TF, DAX-1, and SF-1 were detected in the nuclei of adrenocortical cells, but not in the medulla. In cortisol-producing adenomas causing Cushing syndrome (CS, n=20), CYP17 expression was upregulated (298±2% vs NL 98 ± 4%), whereas expression levels of both COUP-TFs (COUP-TFI, 52±5% vs NL 98±4%; COUP-TFII, 18±4% vs NL 98±4%) and DAX-1 (42±4% vs NL 100±4%) were reduced. In deoxycorticosterone-producing adenomas (DOC, n=2), on the other hand, CYP17 expression was extremely reduced (8 and 12% vs NL 98±4%), whereas DAX-1 expression increased markedly (350 and 360% vs NL 100±4%). Expression levels of SF-1 did not differ between NL (100±8%) and CS (106±10%), but its expression appeared to be decreased in DOC (25 and 20%). These results showed CYP17 expression to be upregulated and downregulated in CS and DOC, respectively, in a manner reciprocal to that of its repressors, COUP-TF and/or DAX-1. In summary, the results indicate that co-localization of COUP-TF, DAX-1, and SF-1 in NL was lost in adrenocortical tumors and that these orphan receptors play an important role in the regulation of steroidogenesis in human adrenals.

AB - Chicken ovalbumin upstream promoter-transcription factor (COUP-TF), DAX-1, and steroidogenic factor-1 (SF-1) are orphan members of the nuclear hormone receptor superfamily. COUP-TF and DAX-1 have been shown to negatively regulate the transcriptional activity of SF-1, a steroidogenic cell-specific activator of various steroidogenic cytochrome P450 genes. We therefore examined the expression levels and immunolocalization of COUP-TF, DAX-1, and SF-1 in human adrenal gland (NL) and adrenocortical adenomas, and compared the results with CYP17 expression levels and its enzyme activities to study their potential correlation with adrenocortical steroidogenesis. In NL (n=10), expressions of COUP-TF, DAX-1, and SF-1 were detected in the nuclei of adrenocortical cells, but not in the medulla. In cortisol-producing adenomas causing Cushing syndrome (CS, n=20), CYP17 expression was upregulated (298±2% vs NL 98 ± 4%), whereas expression levels of both COUP-TFs (COUP-TFI, 52±5% vs NL 98±4%; COUP-TFII, 18±4% vs NL 98±4%) and DAX-1 (42±4% vs NL 100±4%) were reduced. In deoxycorticosterone-producing adenomas (DOC, n=2), on the other hand, CYP17 expression was extremely reduced (8 and 12% vs NL 98±4%), whereas DAX-1 expression increased markedly (350 and 360% vs NL 100±4%). Expression levels of SF-1 did not differ between NL (100±8%) and CS (106±10%), but its expression appeared to be decreased in DOC (25 and 20%). These results showed CYP17 expression to be upregulated and downregulated in CS and DOC, respectively, in a manner reciprocal to that of its repressors, COUP-TF and/or DAX-1. In summary, the results indicate that co-localization of COUP-TF, DAX-1, and SF-1 in NL was lost in adrenocortical tumors and that these orphan receptors play an important role in the regulation of steroidogenesis in human adrenals.

UR - http://www.scopus.com/inward/record.url?scp=0034788474&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034788474&partnerID=8YFLogxK

U2 - 10.1006/mgme.2001.3231

DO - 10.1006/mgme.2001.3231

M3 - Article

C2 - 11592817

AN - SCOPUS:0034788474

VL - 74

SP - 206

EP - 216

JO - Molecular Genetics and Metabolism

JF - Molecular Genetics and Metabolism

SN - 1096-7192

IS - 1-2

ER -