Expression profiles of TCRβ and CD8α mRNA correlate with virus-specific cell-mediated cytotoxic activity in ginbuna crucian carp

Tomonori Somamoto, Yasutoshi Yoshiura, Atsushi Sato, Miki Nakao, Teruyuki Nakanishi, Nobuaki Okamoto, Mitsuru Ototake

研究成果: ジャーナルへの寄稿記事

64 引用 (Scopus)

抄録

Our previous studies have demonstrated that virus-specific cell-mediated cytotoxicity of sensitized leukocytes can be induced using clonal ginbuna crucian carp and their syngeneic cell lines. In the present study, we attempt to determine if virus-specific cytotoxic cell populations of fish express CD8α and TCRβ genes. Leukocytes from ginbuna crucian carp were separated into four fractions by immunomagnetic separation and density gradient centrifugation: Fraction A, leukocytes with a density of 1.08 g/ml (primarily lymphocytes); Fraction B, sIg-negative leukocytes with density of 1.08 g/ml; Fraction C, sIg-positive cells (primarily B-lymphocytes); Fraction D, leukocytes with a density of 1.08-1.09 g/ml (primarily neutrophils). Leukocytes in all fractions from uninfected fish do not exhibit cytotoxic activity against virus-infected syngeneic cells and weakly express CD8α and TCRβ mRNAs. In contrast, leukocytes in fractions A and B from virus-infected fish exhibit a high level of cytotoxic activity and strongly express CD8α and TCRβ mRNAs. In addition, mRNA expressions of CD8α and TCRβ in effector cells are upregulated by cocultivation with virus-infected target cells but not uninfected ones. The present study suggests that fish possess virus-specific cytotoxic cells with phenotype and gene expression pattern similar to those of CTLs in mammals.

元の言語英語
ページ(範囲)370-377
ページ数8
ジャーナルVirology
348
発行部数2
DOI
出版物ステータス出版済み - 5 10 2006

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Carps
Leukocytes
Viruses
Messenger RNA
Fishes
Immunomagnetic Separation
Cercopithecine Herpesvirus 1
Density Gradient Centrifugation
Coculture Techniques
Mammals
Neutrophils
B-Lymphocytes
Lymphocytes
Phenotype
Gene Expression
Cell Line
Population
Genes

All Science Journal Classification (ASJC) codes

  • Virology

これを引用

Expression profiles of TCRβ and CD8α mRNA correlate with virus-specific cell-mediated cytotoxic activity in ginbuna crucian carp. / Somamoto, Tomonori; Yoshiura, Yasutoshi; Sato, Atsushi; Nakao, Miki; Nakanishi, Teruyuki; Okamoto, Nobuaki; Ototake, Mitsuru.

:: Virology, 巻 348, 番号 2, 10.05.2006, p. 370-377.

研究成果: ジャーナルへの寄稿記事

Somamoto, Tomonori ; Yoshiura, Yasutoshi ; Sato, Atsushi ; Nakao, Miki ; Nakanishi, Teruyuki ; Okamoto, Nobuaki ; Ototake, Mitsuru. / Expression profiles of TCRβ and CD8α mRNA correlate with virus-specific cell-mediated cytotoxic activity in ginbuna crucian carp. :: Virology. 2006 ; 巻 348, 番号 2. pp. 370-377.
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