Expression profiling of cumulus cells reveals functional changes during ovulation and central roles of prostaglandin EP2 receptor in cAMP signaling

Shigero Tamba, Rieko Yodoi, Kazushi Morimoto, Tomoaki Inazumi, Mamiko Sukeno, Eri Segi-Nishida, Yasushi Okuno, Gozoh Tsujimoto, Shuh Narumiya, Yukihiko Sugimoto

研究成果: Contribution to journalArticle査読

14 被引用数 (Scopus)

抄録

To understand the role of prostaglandin (PG) receptor EP2 (Ptger2) signaling in ovulation and fertilization, we investigated time-dependent expression profiles in wild-type (WT) and Ptger2-/- cumuli before and after ovulation by using microarrays. We prepared cumulus cells from mice just before and 3, 9 and 14 h after human chorionic gonadotropin injection. Key genes including cAMP-related and epidermal growth factor (EGF) genes, as well as extracellular matrix- (ECM-) related and chemokine genes were up-regulated in WT cumuli at 3 h and 14 h, respectively. Ptger2 deficiency differently affected the expression of many of the key genes at 3 h and 14 h. These results indicate that the gene expression profile of cumulus cells greatly differs before and after ovulation, and in each situation, PGE2-EP2 signaling plays a critical role in cAMP-regulated gene expression in the cumulus cells under physiological conditions.

本文言語英語
ページ(範囲)665-675
ページ数11
ジャーナルBiochimie
92
6
DOI
出版ステータス出版済み - 6 2010
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学

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